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  • KD/KO Validated

GFPT1 Polyclonal antibody

GFPT1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14132-1-AP

Synonyms

GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, HepG2 cells
Positive IHC detected inhuman gliomas tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14132-1-AP targets GFPT1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFPT1 fusion protein Ag5312
Full Name glutamine-fructose-6-phosphate transaminase 1
Calculated Molecular Weight 681 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC045641
Gene Symbol GFPT1
Gene ID (NCBI) 2673
RRIDAB_2110155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFPT1 (glutamine-fructose-6-phosphate transaminase 1) catalyzes the transfer of an amino group from glutamine onto fructose-6-phosphate, yielding glucosamine-6-phosphate (GlcN-6-P) and glutamate. GFPT1 is the key enzyme of the hexosamine pathway and is required for critical events in neuromuscular transmission. Mutations of GFPT1 cause congenital myasthenic syndromes (CMS). (21310273, 25765662)

Protocols

Product Specific Protocols
WB protocol for GFPT1 antibody 14132-1-APDownload protocol
IHC protocol for GFPT1 antibody 14132-1-APDownload protocol
IF protocol for GFPT1 antibody 14132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Authors - Yuan-Li Ai
mouseWB

Cell Metab

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate

Authors - Peng Zhou

Circ Res

Mitochondrial Protein Poldip2 Controls VSMC Differentiated Phenotype by O-Linked GlcNAc Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.

Authors - Felipe Paredes
humanIF

J Clin Invest

Targeting tumor-intrinsic hexosamine biosynthesis sensitizes pancreatic cancer to anti-PD1 therapy.

Authors - Nikita S Sharma
WB

Nat Commun

Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.

Authors - Adela S Oliva Chávez
humanWB

Am J Hum Genet

Hexosamine biosynthetic pathway mutations cause neuromuscular transmission defect.

Authors - Senderek Jan J

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kristian (Verified Customer) (06-07-2021)

Mouse testis were stained for Gfpt1 (green) and DAPI (blue)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse testis
GFPT1 Antibody Immunofluorescence validation (1:200 dilution) in Mouse testis (Cat no:14132-1-AP)