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  • KD/KO Validated

GFPT1 Polyclonal antibody

GFPT1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14132-1-AP

Synonyms

GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, HepG2 cells
Positive IHC detected inhuman gliomas tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14132-1-AP targets GFPT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFPT1 fusion protein Ag5312
Full Name glutamine-fructose-6-phosphate transaminase 1
Calculated molecular weight 681 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC045641
Gene symbol GFPT1
Gene ID (NCBI) 2673
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GFPT1 (glutamine-fructose-6-phosphate transaminase 1) catalyzes the transfer of an amino group from glutamine onto fructose-6-phosphate, yielding glucosamine-6-phosphate (GlcN-6-P) and glutamate. GFPT1 is the key enzyme of the hexosamine pathway and is required for critical events in neuromuscular transmission. Mutations of GFPT1 cause congenital myasthenic syndromes (CMS). (21310273, 25765662)

Protocols

Product Specific Protocols
WB protocol for GFPT1 antibody 14132-1-APDownload protocol
IHC protocol for GFPT1 antibody 14132-1-APDownload protocol
IF protocol for GFPT1 antibody 14132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biochim Biophys Acta Mol Basis Dis

Consumption of a high fat diet promotes protein O-GlcNAcylation in mouse retina via NR4A1-dependent GFAT2 expression.

Authors - Weiwei Dai
mouseWB

Int J Mol Sci

PMID: 32630335

Authors - Luke A Weyrauch
humanWB

Glycobiology

Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.

Authors - Sanna Oikari
  • KD Validated
humanWB

Am J Physiol Cell Physiol

Hydrogen Sulfide Accumulates LDL Receptor Precursor via Downregulating PCSK9 in HepG2 Cells.

Authors - Yong Huang
mouseWB

J Agric Food Chem

Ginsenoside-Rb1 Improved Diabetic Cardiomyopathy through Regulating Calcium Signaling by Alleviating Protein O-GlcNAcylation.

Authors - Linhui Qin
humanWB, IF

Am J Physiol Cell Physiol

Trivalent Chromium Inhibits TSP-1 Expression, Proliferation and O-GlcNAc signaling in Vascular Smooth Muscle Cells in Response to High Glucose in Vitro.

Authors - Rituparna Ganguly