GMF-beta Antibody

GMF-beta Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3B10A2

Cat no : 60062-1-Ig

Synonyms

glia maturation factor beta, GMFB, GMF-beta



Tested Applications

Positive WB detected infetal human brain tissue, pig liver tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60062-1-Ig targets GMF-beta in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GMF-beta fusion protein Ag1104
Full Name glia maturation factor, beta
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC005359
Gene symbol GMFB
Gene ID (NCBI) 2764
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.

Protocols

Product Specific Protocols
WB protocol for GMF-beta antibody 60062-1-IgDownload protocol
IHC protocol for GMF-beta antibody 60062-1-IgDownload protocol
IF protocol for GMF-beta antibody 60062-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseIF

PLoS One

Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.

Authors - Duraisamy Kempuraj
humanIF

J Alzheimers Dis

Co-Localization of Glia Maturation Factor with NLRP3 Inflammasome and Autophagosome Markers in Human Alzheimer's Disease Brain.

Authors - Mohammad Ejaz Ahmed
humanIP

Transl Psychiatry

Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein.

Authors - Brawnie Petrov