RACK1 Antibody

RACK1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 10076-1-AP

Synonyms

GNB2L1, H12.3, HLC 7, PIG21, RACK1



Tested Applications

Positive IHC detected inhuman lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10076-1-AP targets RACK1 in IHC, ELISA applications and shows reactivity with human samples.

Reactivity human
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RACK1 fusion protein Ag0120
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
Calculated molecular weight 36 kDa
Observed molecular weight
GenBank accession number BC019093
Gene symbol GNB2L1
Gene ID (NCBI) 10399
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Publications

SpeciesApplicationTitle
humanWB

Environ Toxicol Pharmacol

4-tert-octylphenol injures motility and viability of human sperm by affecting cAMP-PKA/PKC-tyrosine phosphorylation signals.

Authors - Shaoping Huang