GPT Polyclonal antibody

GPT Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16897-1-AP

Synonyms

AAT1, Alanine aminotransferase 1, ALT1, GPT, GPT 1, GPT1



Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IP detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

16897-1-AP targets GPT in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPT fusion protein Ag10424
Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC018207
Gene symbol GPT
Gene ID (NCBI) 2875
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GPT antibody 16897-1-APDownload protocol
IP protocol for GPT antibody 16897-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cancer Med

Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.

Authors - Xiaoxiao Cai
mouseWB

Mediators Inflamm

Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.

Authors - Shuaiwei Wang
human, mouseWB

J Proteome Res

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Authors - Shizhen Qin
humanWB

Oncotarget

Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.

Authors - Santosh K Bharti
mouseWB

Cell Rep

T Cell Activation Depends on Extracellular Alanine.

Authors - Noga Ron-Harel
WB

Cell Metab

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Authors - Qiuying Chen