• Featured Product
  • KD/KO Validated

GSK3B Antibody

GSK3B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 51065-1-AP

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

51065-1-AP targets GSK3B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glycogen synthase kinase 3 beta
Calculated molecular weight 433aa,48 kDa
Observed molecular weight 46-52 kDa
GenBank accession number BC000251
Gene symbol GSK3B
Gene ID (NCBI) 2932
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the C-terminal of GSK3B.

Protocols

Product Specific Protocols
WB protocol for GSK3B antibody 51065-1-APDownload protocol
IHC protocol for GSK3B antibody 51065-1-APDownload protocol
IF protocol for GSK3B antibody 51065-1-APDownload protocol
IP protocol for GSK3B antibody 51065-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Steroid Biochem Mol Biol

High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.

Authors - Rui Wang
mouseIF

Mol Biol Cell

ILK modulates epithelial polarity and matrix formation in hair follicles.

Authors - Alena Rudkouskaya