HA-Tag Antibody 14 Publications

Mouse Monoclonal| Catalog number: 66006-1-Ig |CloneNo.: 1C1D2

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Freight/Packing

Con: 75 μg/150 μl

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Species specificity:
recombinant protein, human

Positive WB detected in:
Recombinant protein

Positive IP detected in:
Transfected HEK-293 cells

Positive IF detected in:
Transfected HEK-293 cells

Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
IF : 1:10-1:100

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG1

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
HA-Tag

Calculated molecular weight:

Observed molecular weight:

GenBank accession number:

Gene ID (NCBI):

Gene symbol

Synonyms
HA Tag, YPYDVPDYA
Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.


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