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- KD/KO Validated
Hexokinase 1 Polyclonal antibody
Hexokinase 1 Polyclonal Antibody for IHC, IP, WB,ELISA
Cat no : 19662-1-AP
|Positive WB detected in||HEK-293 cells, mouse brain tissue, human brain tissue, human skeletal muscle tissue, mouse heart tissue, rat heart tissue, HepG2 cells, HeLa cells, MCF-7 cells, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19662-1-AP is Hexokinase 1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Hexokinase 1 fusion protein Ag8015|
|Full Name||hexokinase 1|
|Calculated molecular weight||102 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC008730|
|Gene ID (NCBI)||3098|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Arthritis Res Ther
Inhibition of hexokinases holds potential as treatment strategy for rheumatoid arthritis.
Anat Cell Biol
Poly(ADP-ribose) polymerase regulates glycolytic activity in kidney proximal tubule epithelial cells.
Br J Pharmacol
Reprod Fertil Dev
Localisation and function of glucose transporter GLUT1 in chicken (Gallus gallus domesticus) spermatozoa: relationship between ATP production pathways and flagellar motility.
Biochem Biophys Res Commun
The changes in glucose metabolism and cell proliferation in the kidneys of polycystic kidney disease mini-pig models.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (01-30-2020)
Strong, clear band for mouse heart.
SCOTT (Verified Customer) (11-20-2018)
Used 1 in/1000 in 5% BSA TBS-Tween overnight at 4 C rolling. The membrane was blocked in 5% BSA TBS-Tween prior to adding the antibody.