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  • KD/KO Validated

Hexokinase 1 Polyclonal antibody

Hexokinase 1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19662-1-AP

Synonyms

Brain form hexokinase, Hexokinase 1, Hexokinase type I, HK I, HK1, HK1 ta, HK1 tb, HK1 tc, HKI, HXK1



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, human brain tissue, human skeletal muscle tissue, mouse brain tissue, mouse heart tissue, rat heart tissue, Mcf-7 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19662-1-AP is Hexokinase 1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hexokinase 1 fusion protein Ag8015
Full Name hexokinase 1
Calculated molecular weight 102 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC008730
Gene symbol HK1
Gene ID (NCBI) 3098
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Hexokinase 1 antibody 19662-1-APDownload protocol
IHC protocol for Hexokinase 1 antibody 19662-1-APDownload protocol
IP protocol for Hexokinase 1 antibody 19662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Arthritis Res Ther

Inhibition of hexokinases holds potential as treatment strategy for rheumatoid arthritis.

Authors - Guanhua Song
  • KD Validated
pigWB

Anat Cell Biol

Poly(ADP-ribose) polymerase regulates glycolytic activity in kidney proximal tubule epithelial cells.

Authors - Hana Song
pigWB

Br J Pharmacol

PMID: 30515768

Authors - Xiaoying Lian
WB

Biomolecules

PMID: 31480692

Authors - Xixi Guo
humanWB

Biochem Biophys Res Commun

The changes in glucose metabolism and cell proliferation in the kidneys of polycystic kidney disease mini-pig models.

Authors - Xiaoying Lian
ratWB

Biochem Biophys Res Commun

Deoxyelephantopin suppresses hepatic stellate cells activation associated with inhibition of aerobic glycolysis via hedgehog pathway.

Authors - Wei Gao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (01-30-2020)

Strong, clear band for mouse heart.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Heart
FH

SCOTT (Verified Customer) (11-20-2018)

Used 1 in/1000 in 5% BSA TBS-Tween overnight at 4 C rolling. The membrane was blocked in 5% BSA TBS-Tween prior to adding the antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human induced neuronal progenitor derived astrocytes
Hexokinase 1 Antibody Western Blot, validation (1/1000 dilution) in Human induced neuronal progenitor derived astrocytes (Cat no:19662-1-AP)