HLA class I ABC Antibody

HLA class I ABC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15240-1-AP

Synonyms

HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC, HLA-A, MHC class I antigen A*3



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells, mouse thymus tissue, RAW 264.7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15240-1-AP targets HLA class I ABC in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA class I ABC fusion protein Ag7370
Full Name major histocompatibility complex, class I, A
Calculated molecular weight 41 kDa
Observed molecular weight 44 kDa
GenBank accession number BC003069
Gene symbol HLA-A
Gene ID (NCBI) 3105
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
WB protocol for HLA class I ABC antibody 15240-1-APDownload protocol
IHC protocol for HLA class I ABC antibody 15240-1-APDownload protocol
IF protocol for HLA class I ABC antibody 15240-1-APDownload protocol
IP protocol for HLA class I ABC antibody 15240-1-APDownload protocol
FC protocol for HLA class I ABC antibody 15240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Oncol

Long Non-coding RNA LINC02195 as a Regulator of MHC I Molecules and Favorable Prognostic Marker for Head and Neck Squamous Cell Carcinoma.

Authors - Hao Li
humanIHC

Histopathology

The prognostic impact of human leukocyte antigen (HLA) class I antigen abnormalities in salivary gland cancer. A clinicopathological study of 288 cases.

Authors - Müller Maximilian M
mouseWB,RIP

Cancer Med

Noncoding RNA 886 alleviates tumor cellular immunological rejection in host C57BL/C mice.

Authors - Hui Ma

Clin Exp Immunol

Activation of Toll-like receptor 7 signaling in labial salivary glands of primary Sjögren's syndrome patients.

Authors - Toshimasa Shimizu
humanWB

PLoS One

Nef Neutralizes the Ability of Exosomes from CD4+ T Cells to Act as Decoys during HIV-1 Infection.

Authors - Julianne V de Carvalho
humanWB

J Biol Chem

Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.

Authors - Chengfeng Merriman

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Patricia (Verified Customer) (02-25-2020)

I tried probing my WB for 1 hour at RT, but it looked terrible, so I will try an overnight incubation. I've also tried my IP twice, once using 4 ug/mL, and the second time using 10 ug/mL. I had no pulldown either time. I'm quite disappointed.

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000 for WB; 4-10 ug/mL IP
  • Cell Tissue Type: Ovarian cancer
No image submitted with review