|Positive WB detected in||A549 cells, HEK-293 cells, HeLa cells, HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human tonsillitis tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15240-1-AP targets HLA class I ABC in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||HLA class I ABC fusion protein Ag7370|
|Full Name||major histocompatibility complex, class I, A|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||44 kDa|
|GenBank accession number||BC003069|
|Gene ID (NCBI)||3105|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
|Product Specific Protocols|
|WB protocol for HLA class I ABC antibody 15240-1-AP||Download protocol|
|IHC protocol for HLA class I ABC antibody 15240-1-AP||Download protocol|
|IF protocol for HLA class I ABC antibody 15240-1-AP||Download protocol|
|IP protocol for HLA class I ABC antibody 15240-1-AP||Download protocol|
|FC protocol for HLA class I ABC antibody 15240-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Patricia (Verified Customer) (02-25-2020)
I tried probing my WB for 1 hour at RT, but it looked terrible, so I will try an overnight incubation. I've also tried my IP twice, once using 4 ug/mL, and the second time using 10 ug/mL. I had no pulldown either time. I'm quite disappointed.