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HLA-DPB1 Polyclonal antibody

HLA-DPB1 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11298-1-AP

Synonyms

DPB1, HLA DP1B, HLA DPB1, HLA-DPB1, MHC class II antigen DPB1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA-DPB1 Polyclonal antibody (11298-1-AP)

    WB analysis using 11298-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 11298-1-AP (HLA-DPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DPB1 Polyclonal antibody (11298-1-AP)

    IHC staining of human tonsillitis using 11298-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11298-1-AP (HLA-DPB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DPB1 Polyclonal antibody (11298-1-AP)

    IHC staining of human tonsillitis using 11298-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11298-1-AP (HLA-DPB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using HLA-DPB1 Polyclonal antibody (11298-1-AP)

    IHC staining of human spleen using 11298-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11298-1-AP (HLA-DPB1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using HLA-DPB1 Polyclonal antibody (11298-1-AP)

    IHC staining of human spleen using 11298-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11298-1-AP (HLA-DPB1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using HLA-DPB1 Polyclonal antibody (11298-1-AP)

    IF Staining of human tonsillitis using 11298-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HLA-DPB1 antibody (11298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using HLA-DPB1 Polyclonal antibody (11298-1-AP)

    IF Staining of human tonsillitis using 11298-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HLA-DPB1 antibody (11298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of Daudi cells using HLA-DPB1 Polyclonal antibody (11298-1-AP)

    FC experiment of Daudi using 11298-1-AP

    1X10^6 Daudi cells were intracellularly stained with 0.4 ug Anti-Human HLA-DPB1 (11298-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inDaudi cells
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inDaudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

11298-1-AP targets HLA-DPB1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DPB1 fusion protein Ag1815
Full Name major histocompatibility complex, class II, DP beta 1
Calculated Molecular Weight 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC015000
Gene Symbol HLA-DPB1
Gene ID (NCBI) 3115
RRIDAB_2877755
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HLA-DPB1 antibody 11298-1-APDownload protocol
IHC protocol for HLA-DPB1 antibody 11298-1-APDownload protocol
IF protocol for HLA-DPB1 antibody 11298-1-APDownload protocol
FC protocol for HLA-DPB1 antibody 11298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Sci Rep

Histological and contractile changes in the genioglossus muscle after nasal obstruction in growing rats

Authors - Karin Harumi Uchima Koecklin
View Article