Human SERPINA1 ELISA Kit

Sensitivity

0.20 ng/mL

Range

1.56 - 100 ng/mL , 6.25 - 400 ng/mL

Reactivity

Human

Cat no : KE00178

Synonyms

A1A, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, alpha 1-Antitrypsin, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT



Product Information

KE00178 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SERPINA1 ELISA kit is to be used to detect and quantify protein levels of endogenous SERPINA1. The assay recognizes human SERPINA1. An antibody specific for SERPINA1 has been pre-coated onto the microwells. The SERPINA1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SERPINA1 is added to detect the captured SERPINA1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human SERPINA1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Urine, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.20 ng/mL
Range 1.56 - 100 ng/mL , 6.25 - 400 ng/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human serum 99%81%-118%
Human urine 88%77%-104%
cell culture supernatants101%90%-116%

IntraAssay

Samplenmean ( ng/mL)SDCV%
1209.71.010
22048.93.98.1
320210.118.68.8

InterAssay

Samplenmean ( ng/mL)SDCV%
12410.41.110.1
22441.64.19.8
324196.813.26.7

Background Information

SERPINA1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors. It is an acute phase glycoprotein mainly (by 80%) synthesized in human liver and is a major blood protein after albumin and the Immunoglobulins. SERPINA1 plays a key antiinflammatory role to protect tissues against proteolytic digestion by elastase secreted by neutrophils. In patients with carcinomas, it was reported that SERPINA1 promotes invasive and metastatic capacity in lung cancer, gastric cancer, and colorectal carcinomas.Several studies demonstrated that higher levels of SERPINA1 correlate with more advanced cancer stages. Defects in this gene can cause emphysema or liver disease.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms A1A, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, alpha 1-Antitrypsin, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT