Tested Applications
| Positive WB detected in | HEK-293 cells, HAP1 cells, rat liver tissue, HeLa cells, HepG2 cells, NIH/3T3 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 51 publications below |
| IHC | See 8 publications below |
| IF | See 2 publications below |
| IP | See 5 publications below |
| CoIP | See 2 publications below |
Product Information
12332-1-AP targets IDH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IDH1 fusion protein Ag2986 Predict reactive species |
| Full Name | isocitrate dehydrogenase 1 (NADP+), soluble |
| Calculated Molecular Weight | 414 aa, 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC012846 |
| Gene Symbol | IDH1 |
| Gene ID (NCBI) | 3417 |
| RRID | AB_2123159 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75874 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IDH1 antibody 12332-1-AP | Download protocol |
| IHC protocol for IDH1 antibody 12332-1-AP | Download protocol |
| IF protocol for IDH1 antibody 12332-1-AP | Download protocol |
| IP protocol for IDH1 antibody 12332-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature IDH mutation impairs histone demethylation and results in a block to cell differentiation. | ||
Cancer Cell Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation. | ||
Cell Stem Cell PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Umut (Verified Customer) (07-25-2023) | The antibody worked very well for Western Blot in a standard application with an average dilution and exposure.
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