• Featured Product
  • KD/KO Validated

IDH1 Polyclonal antibody

IDH1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12332-1-AP

Synonyms

IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD



Tested Applications

Positive WB detected inmouse liver tissue, DU 145 cells, HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, mouse pancreas tissue, mouse small intestine tissue, NIH/3T3 cells, rat liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman gliomas tissue, human kidney tissue, human liver cancer tissue, human lung tissue, human placenta tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:100
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12332-1-AP targets IDH1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDH1 fusion protein Ag2986
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Calculated molecular weight 414 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC012846
Gene symbol IDH1
Gene ID (NCBI) 3417
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

Protocols

Product Specific Protocols
WB protocol for IDH1 antibody 12332-1-APDownload protocol
IHC protocol for IDH1 antibody 12332-1-APDownload protocol
IF protocol for IDH1 antibody 12332-1-APDownload protocol
IP protocol for IDH1 antibody 12332-1-APDownload protocol
FC protocol for IDH1 antibody 12332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nucleic Acids Res

Insight into novel RNA-binding activities via large-scale analysis of lncRNA-bound proteome and IDH1-bound transcriptome.

Authors - Lichao Liu
humanWB

Br J Cancer

Metabolic reprogramming and Notch activity distinguish between non-small cell lung cancer subtypes.

Authors - Katherine Sellers
humanWB

Proc Natl Acad Sci U S A

LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect.

Authors - Shaoxun Xiang
  • KD Validated
WB

Environ Pollut

Proteomic profile in Perna viridis after exposed to Prorocentrum lima, a dinoflagellate producing DSP toxins.

Authors - Lu Huang
humanWB,IHC

Graefes Arch Clin Exp Ophthalmol

Proteomic analysis of the differences in orbital protein expression in thyroid orbitopathy.

Authors - Cheng Kai-Chun KC

Oncol Rep

HIF-1α inhibits IDH-1 expression in osteosarcoma.

Authors - Deng-Cheng Liu