- Featured Product
- KD/KO Validated
IDH1 Polyclonal antibody
IDH1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 12332-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, DU 145 cells, HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, mouse pancreas tissue, mouse small intestine tissue, NIH/3T3 cells, rat liver tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human gliomas tissue, human kidney tissue, human liver cancer tissue, human lung tissue, human placenta tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:100 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 21 publications below |
| IHC | See 5 publications below |
| IF | See 1 publications below |
Product Information
12332-1-AP targets IDH1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IDH1 fusion protein Ag2986 |
| Full Name | isocitrate dehydrogenase 1 (NADP+), soluble |
| Calculated molecular weight | 414 aa, 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC012846 |
| Gene symbol | IDH1 |
| Gene ID (NCBI) | 3417 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IDH1 antibody 12332-1-AP | Download protocol |
| IHC protocol for IDH1 antibody 12332-1-AP | Download protocol |
| IF protocol for IDH1 antibody 12332-1-AP | Download protocol |
| IP protocol for IDH1 antibody 12332-1-AP | Download protocol |
| FC protocol for IDH1 antibody 12332-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Insight into novel RNA-binding activities via large-scale analysis of lncRNA-bound proteome and IDH1-bound transcriptome. | ||
Br J Cancer Metabolic reprogramming and Notch activity distinguish between non-small cell lung cancer subtypes. | ||
Proc Natl Acad Sci U S A LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect.
| ||
Environ Pollut Proteomic profile in Perna viridis after exposed to Prorocentrum lima, a dinoflagellate producing DSP toxins. | ||
Graefes Arch Clin Exp Ophthalmol Proteomic analysis of the differences in orbital protein expression in thyroid orbitopathy. | ||
