DU 145 cells were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

rat liver tissue were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Immunohistochemical of paraffin-embedded human gliomas using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human gliomas using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human kidney using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human kidney using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12332-1-AP( IDH1 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12332-1-AP( IDH1 Antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human lung using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human lung using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human placenta using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human placenta using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human skin using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human skin using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-IDH1 (IP:12332-1-AP, 3ug; Detection:12332-1-AP 1:600) with MCF-7 cells lysate 2500ug.

Immunofluorescent analysis of HepG2 cells using 12332-1-AP(IDH1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

1X10^6 HeLa cells were stained with 0.2ug IDH1 antibody (12332-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.