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IFIT2 Polyclonal antibody

IFIT2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12604-1-AP

Synonyms

cig42, G10P2, GARG 39, IFI 54, IFI 54K, IFI54, IFIT 2, IFIT2, ISG 54 K, ISG 54K, ISG54



Tested Applications

Positive WB detected inA431 cells, RAW 264.7 cells
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman skin cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12604-1-AP targets IFIT2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFIT2 fusion protein Ag3283
Full Name IFIT 2
Calculated molecular weight 484 aa, 56 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC032839
Gene symbol IFIT2
Gene ID (NCBI) 3433
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFIT2 belongs to the IFIT family. It contains 6 TPR repeats. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT proteins are produced in human body that are supposed to confer immunity against viral infection. These proteins are generally produced during viral infection, IFN treatment, and during pathogen recognition (Pathogen associated molecular pattern recognition) by immune system during infections.

Protocols

Product Specific Protocols
WB protocol for IFIT2 antibody 12604-1-APDownload protocol
IHC protocol for IFIT2 antibody 12604-1-APDownload protocol
IF protocol for IFIT2 antibody 12604-1-APDownload protocol
IP protocol for IFIT2 antibody 12604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

The histone chaperone HIRA promotes the induction of host innate immune defences in response to HSV-1 infection.

Authors - Steven McFarlane
WB

Wellcome Open Res

PMID: 31372503

Authors - Harriet V Mears
humanWB

Cell Rep

Cell Cycle Checkpoints Cooperate to Suppress DNA- and RNA-Associated Molecular Pattern Recognition and Anti-Tumor Immune Responses.

Authors - Jie Chen
humanWB

bioRxiv

ISG15-dependent Activation of the RNA Sensor MDA5 and its Antagonism by the SARS-CoV-2 papain-like protease.

Authors - GuanQun Liu
mouseWB

J Biol Chem

Mouse Ifit1b is a cap1-RNA-binding protein that inhibits mouse coronavirus translation and is regulated by complexing with Ifit1c.

Authors - Harriet V Mears
mouseWB

J Biol Chem

Mouse Ifit1b is a cap1-RNA binding protein which inhibits mouse coronavirus translation and is regulated by complexing with Ifit1c.

Authors - Harriet V Mears