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IFIT2 Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 12604-1-AP

Featured Product KD/KO validated

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

IFIT2 Antibody IHC human skin cancer tissue 12604-1-AP

Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IFIT2 Antibody IHC human skin cancer tissue 12604-1-AP

Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IFIT2 Antibody WB A431 cells 12604-1-AP

WB result of IFIT2 antibody (12604-1-AP, 1:300) with si-IFIT2 and si-Control transfected A431 cells.

IFIT2 Antibody WB A431 cells 12604-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 12604-1-AP( IFIT2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night

IFIT2 Antibody IP mouse skin tissue 12604-1-AP

IP Result of anti-IFIT2 (IP:12604-1-AP, 4ug; Detection:12604-1-AP 1:300) with mouse skin tissue lysate 3200ug.

IFIT2 Antibody IHC human kidney tissue 12604-1-AP

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 12604-1-AP( IFIT2 Antibody) at dilution of 1:50 (under 40x lens)

IFIT2 Antibody IF HepG2 cells 12604-1-AP

Immunofluorescent analysis of HepG2 cells using 12604-1-AP(IFIT2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
A431 cells, RAW 264.7 cells
Positive IP detected in:
mouse skin tissue
Positive IHC detected in:
human skin cancer tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IFIT2 fusion protein Ag3283
Full name:
interferon-induced protein with tetratricopeptide repeats 2
Calculated molecular weight:
484aa,56 kDa
Observed molecular weight:
50-55 kDa
GenBank accession number:
BC032839
Gene ID (NCBI):
3433
Gene symbol
IFIT2
Synonyms
cig42, G10P2, GARG 39, IFI 54, IFI 54K, IFI54, IFIT 2, IFIT2, ISG 54 K, ISG 54K, ISG54

Background

IFIT2 belongs to the IFIT family. It contains 6 TPR repeats. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT proteins are produced in human body that are supposed to confer immunity against viral infection. These proteins are generally produced during viral infection, Interferon (IFN) treatment, and during pathogen recognition (Pathogen associated molecular pattern recognition) by immune system during infections.


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