Tested Applications
| Positive WB detected in | A431 cells, THP-1 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 43 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
12604-1-AP targets IFIT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IFIT2 fusion protein Ag3283 Predict reactive species |
| Full Name | IFIT 2 |
| Calculated Molecular Weight | 484 aa, 56 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC032839 |
| Gene Symbol | IFIT2 |
| Gene ID (NCBI) | 3433 |
| RRID | AB_2864734 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09913 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFIT2 belongs to the IFIT family. It contains 6 TPR repeats. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT proteins are produced in human body that are supposed to confer immunity against viral infection. These proteins are generally produced during viral infection, IFN treatment, and during pathogen recognition (Pathogen associated molecular pattern recognition) by immune system during infections.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFIT2 antibody 12604-1-AP | Download protocol |
| IHC protocol for IFIT2 antibody 12604-1-AP | Download protocol |
| IF protocol for IFIT2 antibody 12604-1-AP | Download protocol |
| IP protocol for IFIT2 antibody 12604-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Nat Immunol A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity. | ||
Nat Microbiol ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity. | ||
Nat Microbiol Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs. | ||
Nucleic Acids Res IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA. |
