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IFITM3 Antibody 51 Publications

Rabbit Polyclonal| Catalog number: 11714-1-AP

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Con: 59 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

IFITM3 Antibody WB HeLa cells 11714-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 11714-1-AP (IFITM3 antibody) at dilution of 1:5000 incubated at 4 degree celsius over night

IFITM3 Antibody IHC human lung cancer tissue 11714-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IFITM3 Antibody IHC human lung cancer tissue 11714-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IFITM3 Antibody IP HeLa cells 11714-1-AP

IP Result of anti-IFITM3 (IP:11714-1-AP, 3ug; Detection:11714-1-AP 1:1500) with HeLa cells lysate 2400ug.

IFITM3 Antibody IF HeLa cells 11714-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 11714-1-AP(IFITM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IFITM3 Antibody FC HeLa cells 11714-1-AP

1X10^6 HeLa cells were stained with 0.2ug IFITM3 antibody (11714-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat, goat
Positive WB detected in:
HeLa cells, mouse colon tissue, HepG2 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human lung cancer tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IFITM3 fusion protein Ag2285
Full name:
interferon induced transmembrane protein 3 (1-8U)
Calculated molecular weight:
133aa,15 kDa
Observed molecular weight:
14 kDa
GenBank accession number:
BC006794
Gene ID (NCBI):
10410
Gene symbol
IFITM3
Synonyms
1 8U, Fragilis, IFITM3, IP15

Background

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.


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