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- KD/KO Validated
IFITM3 Polyclonal antibody
IFITM3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 11714-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse colon tissue, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 23 publications below |
| WB | See 51 publications below |
| IHC | See 5 publications below |
| IF | See 10 publications below |
| IP | See 2 publications below |
| FC | See 5 publications below |
Product Information
11714-1-AP targets IFITM3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | goat, human, mouse, rat, swine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IFITM3 fusion protein Ag2285 |
| Full Name | interferon induced transmembrane protein 3 (1-8U) |
| Calculated molecular weight | 133 aa, 15 kDa |
| Observed molecular weight | 14 kDa |
| GenBank accession number | BC006794 |
| Gene symbol | IFITM3 |
| Gene ID (NCBI) | 10410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM3 antibody 11714-1-AP | Download protocol |
| IHC protocol for IFITM3 antibody 11714-1-AP | Download protocol |
| IF protocol for IFITM3 antibody 11714-1-AP | Download protocol |
| IP protocol for IFITM3 antibody 11714-1-AP | Download protocol |
| FC protocol for IFITM3 antibody 11714-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Chem Biol IFITM3 directly engages and shuttles incoming virus particles to lysosomes.
| ||
Int J Biol Macromol Construction, expression and antiviral activity analysis of recombinant adenovirus expressing human IFITM3 in vitro. | ||
MBio Vpr Enhances HIV-1 Env Processing and Virion Infectivity in Macrophages by Modulating TET2-Dependent IFITM3 Expression.
| ||
Cell Rep Cell Lineage Tracing Identifies Hormone-Regulated and Wnt-Responsive Vaginal Epithelial Stem Cells. | ||
Cell Chem Biol Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.
| ||
Res Vet Sci Infection of goats with goatpox virus triggers host antiviral defense through activation of innate immune signaling. |
