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Tested Applications
| Positive WB detected in | human testis tissue, K-562 cells |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| ELISA | See 1 publications below |
Product Information
20673-1-AP targets IL-17RB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | interleukin 17 receptor B |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 56-60 kDa |
| GenBank Accession Number | NM_018725 |
| Gene Symbol | IL-17RB |
| Gene ID (NCBI) | 55540 |
| RRID | AB_10700011 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRM6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL17RB, also named as CRL4, EVI27 and IL17BR, is a receptor for the proinflammatory cytokines IL17B and IL17E. IL17RB may play a role in controlling the growth and/or differentiation of hematopoietic cells. IL17RB has two isoforms with MW 56 kDa (isoform 1) and 32 kDa (isoform 2). The antibody is specific to IL17RB.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IL-17RB antibody 20673-1-AP | Download protocol |
| IHC protocol for IL-17RB antibody 20673-1-AP | Download protocol |
| WB protocol for IL-17RB antibody 20673-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Induction of IL-25 secretion from tumour-associated fibroblasts suppresses mammary tumour metastasis. | ||
Cell Rep Schwann cell promotes macrophage recruitment through IL-17B/IL-17RB pathway in injured peripheral nerves | ||
Respir Res IL-25 induces airway remodeling in asthma by orchestrating the phenotypic changes of epithelial cell and fibrocyte | ||
Front Immunol Interleukin-25-Mediated-IL-17RB Upregulation Promotes Cutaneous Wound Healing in Diabetic Mice by Improving Endothelial Cell Functions. | ||
Arch Toxicol Fluoride induces apoptosis and autophagy through the IL-17 signaling pathway in mice hepatocytes. | ||
