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  • KD/KO Validated

IL-6 Polyclonal antibody

IL-6 Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IF, FC, IHC, ELISA, Neutralization, Cell treatment

Conjugate

Unconjugated

Cat no : 21865-1-AP

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6



Tested Applications

Positive WB detected inLPS and Protein transport inhibitor treated HUVEC cells
Positive FC detected inLPS and Brefeldin A treated HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21865-1-AP targets IL-6 in WB, IF, FC, IHC, ELISA, Neutralization, Cell treatment applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, goat, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-6 fusion protein Ag10565
Full Name interleukin 6 (interferon, beta 2)
Calculated Molecular Weight 212 aa, 24 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC015511
Gene Symbol IL6
Gene ID (NCBI) 3569
RRIDAB_11142677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Protocols

Product Specific Protocols
WB protocol for IL-6 antibody 21865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
IF

Cancer Cell

Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.

Authors - Yansen Xiao
IHC

Bioact Mater

A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds

Authors - Shunlai Shang

ACS Cent Sci

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Authors - Juan Zhang

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
human

Mol Ther

Epsin1-mediated exosomal sorting of Dll4 modulates the tubular-macrophage crosstalk in diabetic nephropathy

Authors - Jia-Lu Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (04-19-2023)

Lowest most band corresponds to IL-6

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS
IL-6 Antibody Western Blot validation (1:1000 dilution) in U2OS (Cat no:21865-1-AP)
FH

Elizabeth (Verified Customer) (12-03-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse