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  • Featured Product
  • KD/KO Validated

IPO13 Polyclonal antibody

IPO13 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11696-2-AP

Synonyms

IMP13, importin 13, IPO13, KAP13, Karyopherin 13, KIAA0724, LGL2, Ran binding protein 13, RANBP13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IPO13 Polyclonal antibody (11696-2-AP)

    WB analysis using 11696-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11696-2-AP (IPO13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using IPO13 Polyclonal antibody (11696-2-AP)

    WB analysis of human brain using 11696-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11696-2-AP (IPO13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using IPO13 Polyclonal antibody (11696-2-AP)

    IHC staining of human gliomas using 11696-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11696-2-AP (IPO13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IPO13 Polyclonal antibody (11696-2-AP)

    IF Staining of HeLa cells using 11696-2-AP

    IF result in Hela cell from Dr. Ralph Kehlenbach showed Nuclear and Cytoplasma stain.

Tested Applications

Positive WB detected inMCF-7 cells, human brain tissue, Neura-2a cells, mouse lung
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11696-2-AP targets IPO13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IPO13 fusion protein Ag2317
Full Name importin 13
Calculated Molecular Weight 963 aa, 108 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC008194
Gene Symbol IPO13
Gene ID (NCBI) 9670
RRIDAB_2127672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IPO13 antibody 11696-2-APDownload protocol
IHC protocol for IPO13 antibody 11696-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IP

Nat Commun

Nuclear transporter Importin-13 plays a key role in the oxidative stress transcriptional response.

Authors - K A Gajewska
  • KD Validated
View Article
humanWB,IHC,IF

Stem Cells

Importin 13 serves as a potential marker for corneal epithelial progenitor cells.

Authors - Wang Hua H
View Article
mouseWB

Data Brief

Transcriptomic profile dataset of embryonic stem cells (Wild-type and IPO13-Knock Out) with and without oxidative stress.

Authors - Katarzyna A Gajewska
  • KO Validated
View Article
humanWB

Cells

The Nuclear Transporter Importin 13 Can Regulate Stress-Induced Cell Death through the Clusterin/KU70 Axis

Authors - Katarzyna A Gajewska
  • KD Validated
View Article