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ISM2 Polyclonal antibody

ISM2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21540-1-AP

Synonyms

ISM2, Isthmin 2, isthmin 2 homolog (zebrafish), TAIL1, THSD3

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using ISM2 Polyclonal antibody (21540-1-AP)

    WB analysis of L02 using 21540-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21540-1-AP (ISM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ISM2 Polyclonal antibody (21540-1-AP)

    WB analysis of mouse kidney using 21540-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21540-1-AP (ISM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using ISM2 Polyclonal antibody (21540-1-AP)

    WB analysis of mouse skin using 21540-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 21540-1-AP (ISM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using ISM2 Polyclonal antibody (21540-1-AP)

    WB analysis of mouse pancreas using 21540-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21540-1-AP (ISM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ISM2 Polyclonal antibody (21540-1-AP)

    IHC staining of human placenta using 21540-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21540-1-AP (ISM2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ISM2 Polyclonal antibody (21540-1-AP)

    IHC staining of human placenta using 21540-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21540-1-AP (ISM2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ISM2 Polyclonal antibody (21540-1-AP)

    IHC staining of human kidney using 21540-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21540-1-AP (ISM2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ISM2 Polyclonal antibody (21540-1-AP)

    IHC staining of human kidney using 21540-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21540-1-AP (ISM2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using ISM2 Polyclonal antibody (21540-1-AP)

    IF Staining of L02 using 21540-1-AP

    Immunofluorescent analysis of L02 cells using 21540-1-AP (ISM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inL02 cells, mouse kidney tissue, mouse pancreas tissue, mouse skin tissue
Positive IHC detected inhuman placenta tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21540-1-AP is ISM2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISM2 fusion protein Ag16187
Full Name isthmin 2 homolog (zebrafish)
Calculated Molecular Weight 571 aa, 64 kDa
Observed Molecular Weight 65 kDa, 55 kDa
GenBank Accession NumberBC101020
Gene Symbol ISM2
Gene ID (NCBI) 145501
RRIDAB_2878880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ISM2 antibody 21540-1-APDownload protocol
IHC protocol for ISM2 antibody 21540-1-APDownload protocol
IF protocol for ISM2 antibody 21540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols