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Integrin Alpha 3 Monoclonal antibody

Integrin Alpha 3 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3F9G4

Cat no : 66070-1-Ig

Synonyms

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a



Tested Applications

Positive WB detected inPC-3 cells, A431 cells, HepG2 cells, A549 cells, mouse brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66070-1-Ig is Integrin Alpha 3 Fusion Protein expressed in E. coli.

Tested Reactivity human, pig, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Integrin Alpha 3 fusion protein Ag17293
Full Name integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
Calculated molecular weight 1066 aa, 119 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC150190
Gene symbol ITGA3
Gene ID (NCBI) 3675
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

Protocols

Product Specific Protocols
WB protocol for Integrin Alpha 3 antibody 66070-1-IgDownload protocol
IHC protocol for Integrin Alpha 3 antibody 66070-1-IgDownload protocol
FC protocol for Integrin Alpha 3 antibody 66070-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Int J Oncol

FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.

Authors - Junfen Xu
humanWB, IF

Mol Cell Proteomics

Hypoxia-induced changes to integrin alpha 3 glycosylation facilitate invasion in epidermoid carcinoma cell line A431.

Authors - Yan Ren