|Positive WB detected in||A549 cells, 3T3-L1 cells, COS-7 cells, HeLa cells, human placenta tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Sample-dependent, check data in validation data gallery|
10569-1-AP targets Integrin Alpha-5 in WB, IF applications and shows reactivity with human, mouse, monkey samples.
|Tested Reactivity||human, mouse, monkey|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Integrin Alpha-5 fusion protein Ag0860|
|Full Name||integrin, alpha 5 (fibronectin receptor, alpha polypeptide)|
|Calculated molecular weight||115 kDa|
|Observed molecular weight||135-150 kDa|
|GenBank accession number||BC008786|
|Gene ID (NCBI)||3678|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form a α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 play roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (02-04-2019)
Excellent. In WB, it gives a protein band at the expected molecular weight, and this band was decreased during adipocyte differentiation, which is consistent with those reported in literature. In IF, it labels endogenous protein at the cell surface, as that reported in literature.