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Integrin Alpha-5 Polyclonal antibody
Integrin Alpha-5 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, monkey and More (1)
Applications
WB, IF
Conjugate
Unconjugated
Cat no : 10569-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, 3T3-L1 cells, COS-7 cells, HeLa cells, human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 13 publications below |
| IF | See 3 publications below |
Product Information
10569-1-AP targets Integrin Alpha-5 in WB, IF applications and shows reactivity with human, mouse, monkey samples.
| Tested Reactivity | human, mouse, monkey |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Integrin Alpha-5 fusion protein Ag0860 |
| Full Name | integrin, alpha 5 (fibronectin receptor, alpha polypeptide) |
| Calculated molecular weight | 115 kDa |
| Observed molecular weight | 135-150 kDa |
| GenBank accession number | BC008786 |
| Gene symbol | ITGA5 |
| Gene ID (NCBI) | 3678 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form a α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 play roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Alpha-5 antibody 10569-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Genet Genomics Identification of rare variants in cardiac sodium channel β4-subunit gene SCN4B associated with ventricular tachycardia. | ||
Oncotarget Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma. | ||
J Immunol Res Ursolic Acid Regulates Intestinal Microbiota and Inflammatory Cell Infiltration to Prevent Ulcerative Colitis. | ||
Exp Ther Med Role of vasodilator-stimulated phosphoprotein in RANKL-differentiated murine macrophage RAW264.7 cells: Modulation of NF-κB, c-Fos and NFATc1 transcription factors. | ||
Biochem Biophys Res Commun Sine oculis homeobox homolog 1 promotes α5β1-mediated invasive migration and metastasis of cervical cancer cells. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Boyan (Verified Customer) (02-04-2019) | Excellent. In WB, it gives a protein band at the expected molecular weight, and this band was decreased during adipocyte differentiation, which is consistent with those reported in literature. In IF, it labels endogenous protein at the cell surface, as that reported in literature.
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