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Integrin alpha-5 Antibody
Integrin alpha-5 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, monkey and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 10569-1-AP
Synonyms
CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, human placenta tissue, COS-7 cells, 3T3-L1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 8 publications below |
| IF | See 2 publications below |
Product Information
10569-1-AP targets Integrin alpha-5 in WB, IF, ELISA applications and shows reactivity with human, mouse, monkey samples.
| Tested Reactivity | human, mouse, monkey |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Integrin alpha-5 fusion protein Ag0860 |
| Full Name | integrin, alpha 5 (fibronectin receptor, alpha polypeptide) |
| Calculated molecular weight | 115 kDa |
| Observed molecular weight | 135-150 kDa |
| GenBank accession number | BC008786 |
| Gene symbol | ITGA5 |
| Gene ID (NCBI) | 3678 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin alpha-5 antibody 10569-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Genet Genomics Identification of rare variants in cardiac sodium channel β4-subunit gene SCN4B associated with ventricular tachycardia. | ||
Oncotarget Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma. | ||
Biochem Biophys Res Commun Sine oculis homeobox homolog 1 promotes α5β1-mediated invasive migration and metastasis of cervical cancer cells. | ||
Cancer Cell Int Silencing Formin-like 2 inhibits growth and metastasis of gastric cancer cells through suppressing internalization of integrins. | ||
PLoS One CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Boyan (Verified Customer) (02-04-2019) | Excellent. In WB, it gives a protein band at the expected molecular weight, and this band was decreased during adipocyte differentiation, which is consistent with those reported in literature. In IF, it labels endogenous protein at the cell surface, as that reported in literature.
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