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ITK Monoclonal antibody

ITK Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1F9D8

Cat no : 67703-1-Ig

Synonyms

EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk

Validation Data Gallery

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  • Western Blot (WB) analysis of PBMC using ITK Monoclonal antibody (67703-1-Ig)

    WB analysis of PBMC using 67703-1-Ig

    PBMC cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ITK Monoclonal antibody (67703-1-Ig)

    WB analysis of Jurkat using 67703-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ITK Monoclonal antibody (67703-1-Ig)

    IF Staining of Jurkat using 67703-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ITK antibody (67703-1-Ig, Clone: 1F9D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using ITK Monoclonal antibody (67703-1-Ig)

    FC experiment of Jurkat using 67703-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human ITK (67703-1-Ig, Clone:1F9D8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 90% MeOH .

Tested Applications

Positive WB detected inJurkat cells, PBMC cells
Positive IF detected inJurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67703-1-Ig targets ITK in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ITK fusion protein Ag17087
Full Name IL2-inducible T-cell kinase
Calculated Molecular Weight 620 aa, 72 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC109077
Gene Symbol ITK
Gene ID (NCBI) 3702
RRIDAB_2882895
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITK is a member of Tec family (BTK, ITK, Tec, BMX and RLK), which expressed in normal T-lymphocytes and T-cell associated hematopoietic malignancies and have confirmed its critical role in regulating T lymphocyte function in EBV-driven lymphoproliferative disease and immune-mediated disorders. Tec kinase family members shares similarities structure, consisting of PH domain, SH3 domain, SH2 domain and kinase domain. Loss of function mutations in ITK results in hypogammaglobulinemia and CD4+ T cell loss in humans, and the patients often present with EBV-associated B cell lymphoproliferative syndrome (PMID: 30814910, PMID: 31025232).

Protocols

Product Specific Protocols
WB protocol for ITK antibody 67703-1-IgDownload protocol
IF protocol for ITK antibody 67703-1-IgDownload protocol
FC protocol for ITK antibody 67703-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols