KRAS-2B-specific Antibody

KRAS-2B-specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16155-1-AP

Synonyms

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2B, KRAS2B speicifc, KRAS4B, Krasa, Krasb, NS3, RASK2



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse brain tissue, mouse kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16155-1-AP targets KRAS-2B-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 21.4 kDa
Observed molecular weight 21 kDa
GenBank accession number NM_004985
Gene symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAS gene family encodes very similar 21KD proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2B.

Protocols

Product Specific Protocols
WB protocol for KRAS-2B-specific antibody 16155-1-APDownload protocol
IHC protocol for KRAS-2B-specific antibody 16155-1-APDownload protocol
IF protocol for KRAS-2B-specific antibody 16155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christopher (Verified Customer) (12-14-2018)

Antibody is very specific but also quite weak. Maybe you can increase the primary antibody dilution to 1:500 to increase signal. There is no unspecific recognition of H-Ras or N-Ras (see picture). Also endogenous Ras only exhibits one band and not two which is a hint for the recognition of endogenous N-Ras next to K-Ras. In immunofluorescence experiments antibody works awesome at a dilution of 1:100 for pancreatic cancer cell lines. In total I'm very satisfied, but you have to use more antibody than written in the data sheet.

  • Applications: Western Blot,Immunofluorescence,Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras
KRAS-2B-specific Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras (Cat no:16155-1-AP)