L-VEGFA Antibody

L-VEGFA Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22341-1-AP

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF



Tested Applications

Positive WB detected inMCF-7 cells, SH-SY5Y cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 22341-1-AP is L-VEGFA Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L-VEGFA fusion protein Ag17831
Full Name vascular endothelial growth factor A
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC065522
Gene symbol VEGFA
Gene ID (NCBI) 7422
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1).

Protocols

Product Specific Protocols
WB protocol for L-VEGFA antibody 22341-1-APDownload protocol
IHC protocol for L-VEGFA antibody 22341-1-APDownload protocol
IF protocol for L-VEGFA antibody 22341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Tumour Biol

Evodiamine exerts anti-tumor effects against hepatocellular carcinoma through inhibiting β-catenin-mediated angiogenesis.

Authors - Le Shi
mouseWB

Life Sci

Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy.

Authors - Jing Zou
humanWB

Mol Carcinog

PMID: 28833603

Authors - Hui-Hui Zhang