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LIF Polyclonal antibody

LIF Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26757-1-AP

Synonyms

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

Validation Data Gallery

  • WB analysis of Jurkat using 26757-1-AP

    WB analysis of Jurkat using 26757-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 26757-1-AP

    IHC staining of human lung cancer using 26757-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 26757-1-AP

    IHC staining of human lung cancer using 26757-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26757-1-AP is LIF Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIF fusion protein Ag25076
Full Name leukemia inhibitory factor (cholinergic differentiation factor)
Calculated molecular weight 202 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC093733
Gene symbol LIF
Gene ID (NCBI) 3976
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

Protocols

Product Specific Protocols
WB protocol for LIF antibody 26757-1-APDownload protocol
IHC protocol for LIF antibody 26757-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Int J Oncol

MACC1 overexpression in carcinoma‑associated fibroblasts induces the invasion of lung adenocarcinoma cells via paracrine signaling.

Authors - Zhuoshi Li
View Article
humanIHC

J Gynecol Obstet Hum Reprod

Anti-phospholipid antibody may reduce endometrial receptivity during the window of embryo implantation.

Authors - Xiaofang Tan
View Article
humanIHC

Int J Cancer

Leukemia inhibitory factor is a novel biomarker to predict lymph node and distant metastasis in pancreatic cancer.

Authors - Wenna Jiang
View Article
humanWB

Hum Reprod

The depot GnRH agonist protocol improves the live birth rate per fresh embryo transfer cycle, but not the cumulative live birth rate in normal responders: a randomized controlled trial and molecular mechanism study.

Authors - Bei Xu
View Article