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LIF Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 26757-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

LIF Antibody WB Jurkat cells 26757-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 26757-1-AP( LIF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

LIF Antibody IHC human lung cancer tissue 26757-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP( LIF Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

LIF Antibody IHC human lung cancer tissue 26757-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP( LIF Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human
Positive WB detected in:
Jurkat cells, MCF-7 cells
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
LIF fusion protein Ag25076
Full name:
leukemia inhibitory factor (cholinergic differentiation factor)
Calculated molecular weight:
202aa,22 kDa
Observed molecular weight:
22 kDa
GenBank accession number:
BC093733
Gene ID (NCBI):
3976
Gene symbol
LIF
Synonyms
CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

Background

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.


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