|Positive WB detected in||Jurkat cells, MCF-7 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 26757-1-AP is LIF Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||LIF fusion protein Ag25076|
|Full Name||leukemia inhibitory factor (cholinergic differentiation factor)|
|Calculated molecular weight||202 aa, 22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC093733|
|Gene ID (NCBI)||3976|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.
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Leukemia inhibitory factor is a novel biomarker to predict lymph node and distant metastasis in pancreatic cancer.
The depot GnRH agonist protocol improves the live birth rate per fresh embryo transfer cycle, but not the cumulative live birth rate in normal responders: a randomized controlled trial and molecular mechanism study.