LIF Polyclonal antibody
LIF Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 26757-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue, mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse embryo tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
Product Information
The immunogen of 26757-1-AP is LIF Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LIF fusion protein Ag25076 |
| Full Name | leukemia inhibitory factor (cholinergic differentiation factor) |
| Calculated molecular weight | 202 aa, 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC093733 |
| Gene symbol | LIF |
| Gene ID (NCBI) | 3976 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LIF antibody 26757-1-AP | Download protocol |
| IHC protocol for LIF antibody 26757-1-AP | Download protocol |
| IF protocol for LIF antibody 26757-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Oncol MACC1 overexpression in carcinoma‑associated fibroblasts induces the invasion of lung adenocarcinoma cells via paracrine signaling. | ||
J Gynecol Obstet Hum Reprod Anti-phospholipid antibody may reduce endometrial receptivity during the window of embryo implantation. | ||
Int J Cancer Leukemia inhibitory factor is a novel biomarker to predict lymph node and distant metastasis in pancreatic cancer. | ||
Hum Reprod The depot GnRH agonist protocol improves the live birth rate per fresh embryo transfer cycle, but not the cumulative live birth rate in normal responders: a randomized controlled trial and molecular mechanism study. |
