LIF Polyclonal antibody, PBS Only

• 

  WB analysis of Jurkat using 26757-1-AP (same clone as 26757-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 26757-1-AP (same clone as 26757-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 26757-1-AP (same clone as 26757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 26757-1-AP (same clone as 26757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using LIF antibody (26757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 26757-1-AP (same clone as 26757-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LIF antibody (26757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

LIF Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 26757-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor

---

Product Information

26757-1-PBS targets LIF in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIF fusion protein Ag25076
• Full Name: leukemia inhibitory factor (cholinergic differentiation factor)
• Calculated Molecular Weight: 202 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC093733
• Gene Symbol: LIF
• Gene ID (NCBI): 3976
• RRID: AB_2880624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P15018
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.