LRRC8A/SWELL1 Polyclonal antibody

LRRC8A/SWELL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17155-1-AP

Synonyms

KIAA1437, LRRC8, LRRC8A, SWELL1



Tested Applications

Positive WB detected inrat lung tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

17155-1-AP targets LRRC8A/SWELL1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC8A/SWELL1 fusion protein Ag10062
Full Name leucine rich repeat containing 8 family, member A
Calculated Molecular Weight 810 aa, 94 kDa
Observed Molecular Weight94 kDa
GenBank Accession NumberBC051322
Gene Symbol LRRC8A
Gene ID (NCBI) 56262
RRIDAB_2918050
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRRC8A, also named as SWELL1, KIAA1437 and LRRC8, belongs to the LRRC8 family. LRRC8A is a ubiquitous and integral component of the VRAC and has been reported to be required for regulation of cell volume. It indicates that LRRC8A plays a role in regulating the flow of ions, especially anions, into and out of cells (PMID: 32079488). Additionally, there have been several reports that LRRC8A is critical for differentiation of adipocytes and lymphocytes (PMID: 31323188). The molecular weight of LRRC8A is 94 kDa.

Protocols

Product Specific Protocols
WB protocol for LRRC8A/SWELL1 antibody 17155-1-APDownload protocol
IHC protocol for LRRC8A/SWELL1 antibody 17155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Biol Sci

Upregulation of LRRC8A by m5C modification-mediated mRNA stability suppresses apoptosis and facilitates tumorigenesis in cervical cancer

Authors - Yanjie Chen