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Lysozyme Monoclonal antibody

Lysozyme Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G5A8

Cat no : 66456-1-Ig

Synonyms

1,4 beta N acetylmuramidase C, lysozyme, lysozyme (renal amyloidosis), Lysozyme C, LYZ, LZM

Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using Lysozyme Monoclonal antibody (66456-1-Ig)

    WB analysis of human spleen using 66456-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spleen tissue using Lysozyme Monoclonal antibody (66456-1-Ig)

    WB analysis of pig spleen using 66456-1-Ig

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using Lysozyme Monoclonal antibody (66456-1-Ig)

    WB analysis of rat spleen using 66456-1-Ig

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using Lysozyme Monoclonal antibody (66456-1-Ig)

    WB analysis of RAW 264.7 using 66456-1-Ig

    LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Lysozyme Monoclonal antibody (66456-1-Ig)

    IHC staining of human colon using 66456-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Lysozyme Monoclonal antibody (66456-1-Ig)

    IHC staining of human colon using 66456-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman spleen tissue, RAW 264.7 cells, pig spleen tissue, rat spleen tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66456-1-Ig targets Lysozyme in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Lysozyme fusion protein Ag6917
Full Name lysozyme (renal amyloidosis)
Calculated Molecular Weight 17 kDa
Observed Molecular Weight15-17 kDa
GenBank Accession NumberBC004147
Gene Symbol LYZ
Gene ID (NCBI) 4069
RRIDAB_2881825
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

Protocols

Product Specific Protocols
WB protocol for Lysozyme antibody 66456-1-IgDownload protocol
IHC protocol for Lysozyme antibody 66456-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Sox10 escalates vascular inflammation by mediating vascular smooth muscle cell transdifferentiation and pyroptosis in neointimal hyperplasia

Authors - Xin Xu
View Article