Lysozyme Monoclonal antibody, PBS Only

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  WB analysis of human spleen using 66456-1-Ig (same clone as 66456-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66456-1-Ig (same clone as 66456-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 66456-1-Ig (same clone as 66456-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66456-1-Ig (same clone as 66456-1-PBS)

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66456-1-Ig (same clone as 66456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66456-1-Ig (same clone as 66456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66456-1-PBS in a different storage buffer formulation.

Lysozyme Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66456-1-PBS

Clone No.2G5A8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

LYZ, 1,4-beta-N-acetylmuramidase C, 2G5A8, EC:3.2.1.17, Lysozyme C

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Product Information

66456-1-PBS targets Lysozyme in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6917

  Product name: Recombinant human Lysozyme protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 24-148 aa of BC004147

  Sequence: CELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV

• Full Name: lysozyme (renal amyloidosis)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC004147
• Gene Symbol: Lysozyme
• Gene ID (NCBI): 4069
• RRID: AB_2881825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61626
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.