MAGEA3 Monoclonal antibody
MAGEA3 Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1A9F3
Cat no : 60054-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, fetal human brain tissue, human testis tissue, rat brain tissue |
Positive IHC detected in | human lung cancer tissue, human oesophagus cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
Product Information
60054-1-Ig targets MAGEA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | MAGEA3 fusion protein Ag0265 |
Full Name | melanoma antigen family A, 3 |
Calculated Molecular Weight | 314 aa, 35 kDa |
Observed Molecular Weight | 35-55 kDa |
GenBank Accession Number | BC000340 |
Gene Symbol | MAGEA3 |
Gene ID (NCBI) | 4102 |
RRID | AB_2138124 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MAGEA3 antibody 60054-1-Ig | Download protocol |
IHC protocol for MAGEA3 antibody 60054-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
PLoS One MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation. | ||
J Transl Med Immunization with a multi-antigen targeted DNA vaccine eliminates chemoresistant pancreatic cancer by disrupting tumor-stromal cell crosstalk |