MAGEA3 Monoclonal antibody

MAGEA3 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat, pig







Cat no : 60054-1-Ig


Antigen MZ2 D, Cancer/testis antigen 1.3, CT1.3, HIP8, HYPD, MAGE 3 antigen, MAGE3, MAGEA3, MAGE-A3, MAGEA6, melanoma antigen family A, 3, Melanoma associated antigen 3

Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, human testis tissue, rat brain tissue
Positive IHC detected inhuman lung cancer tissue, human oesophagus cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

60054-1-Ig targets MAGEA3 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAGEA3 fusion protein Ag0265
Full Name melanoma antigen family A, 3
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 35-55 kDa
GenBank accession numberBC000340
Gene symbol MAGEA3
Gene ID (NCBI) 4102
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Product Specific Protocols
WB protocol for MAGEA3 antibody 60054-1-IgDownload protocol
IHC protocol for MAGEA3 antibody 60054-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



PLoS One

MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation.

Authors - Aaron Chen