MAGEA3 Antibody

MAGEA3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Publications

0

CloneNo.

1A9F3

Cat no : 60054-1-Ig

Synonyms

Antigen MZ2 D, Cancer/testis antigen 1.3, CT1.3, HIP8, HYPD, MAGE 3 antigen, MAGE3, MAGEA3, MAGE-A3, MAGEA6, melanoma antigen family A, 3, Melanoma associated antigen 3



Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, human testis tissue, rat brain tissue
Positive IHC detected inhuman lung cancer tissue, human oesophagus cancer tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60054-1-Ig targets MAGEA3 in IHC, WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAGEA3 fusion protein Ag0265
Full Name melanoma antigen family A, 3
Calculated molecular weight 314aa, 35 kDa
Observed molecular weight 35-55 kDa
GenBank accession number BC000340
Gene symbol MAGEA3
Gene ID (NCBI) 4102
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAGEA3 antibody 60054-1-IgDownload protocol
IHC protocol for MAGEA3 antibody 60054-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols