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MCEE Polyclonal antibody

MCEE Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16499-1-AP

Synonyms

DL methylmalonyl CoA racemase, GLOD2, MCEE, methylmalonyl CoA epimerase

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MCEE Polyclonal antibody (16499-1-AP)

    WB analysis using 16499-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16499-1-AP (MCEE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using MCEE Polyclonal antibody (16499-1-AP)

    WB analysis of human heart using 16499-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16499-1-AP (MCEE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using MCEE Polyclonal antibody (16499-1-AP)

    WB analysis of human liver using 16499-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16499-1-AP (MCEE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using MCEE Polyclonal antibody (16499-1-AP)

    WB analysis of human heart using 16499-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16499-1-AP (MCEE antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MCEE Polyclonal antibody (16499-1-AP)

    IF Staining of HepG2 using 16499-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16499-1-AP (MCEE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, human heart tissue, human liver tissue, rat liver tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16499-1-AP targets MCEE in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCEE fusion protein Ag9665
Full Name methylmalonyl CoA epimerase
Calculated Molecular Weight 176 aa, 19 kDa
Observed Molecular Weight 15-19 kDa
GenBank Accession NumberBC020825
Gene Symbol MCEE
Gene ID (NCBI) 84693
RRIDAB_2143812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MCEE antibody 16499-1-APDownload protocol
IF protocol for MCEE antibody 16499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Metab

Altered propionate metabolism contributes to tumour progression and aggressiveness.

Authors - Ana P Gomes
View Article
humanWB

Biochim Biophys Acta Mol Basis Dis

ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis

Authors - Lan Wang
View Article