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  • KD/KO Validated

Melanophilin Polyclonal antibody

Melanophilin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10338-1-AP

Synonyms

Exophilin 3, melanophilin, MLPH, SLAC2 A, SLAC2A, Synaptotagmin like protein 2a



Tested Applications

Positive WB detected inA375 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10338-1-AP targets Melanophilin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Melanophilin fusion protein Ag0316
Full Name melanophilin
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa, 80 kDa
GenBank accession numberBC001653
Gene symbol MLPH
Gene ID (NCBI) 79083
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

Protocols

Product Specific Protocols
WB protocol for Melanophilin antibody 10338-1-APDownload protocol
IHC protocol for Melanophilin antibody 10338-1-APDownload protocol
IF protocol for Melanophilin antibody 10338-1-APDownload protocol
IP protocol for Melanophilin antibody 10338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomolecules

Identification of MicroRNA Targeting Mlph and Affecting Melanosome Transport.

Authors - Jeong Ah Lee
  • KD Validated
humanWB

Mol Genet Metab

A novel missense mutation (G43S) in the switch I region of Rab27A causing Griscelli syndrome.

Authors - Westbroek Wendy W
humanWB

Onco Targets Ther

MLPH Accelerates the Epithelial-Mesenchymal Transition in Prostate Cancer.

Authors - Tianbiao Zhang
  • KD Validated
humanIHC

J Gastroenterol

Differential gene expression in normal esophagus and Barrett's esophagus.

Authors - Jacob Wang
mouseWB

J Dermatol Sci

16-Kauren-2-beta-18,19-triol inhibits melanosome transport in melanocytes by down-regulation of melanophilin expression.

Authors - Cheol Hwan Myung
humanWB

Sci Rep

Inhibitory effect of 2-methyl-naphtho[1,2,3-de]quinolin-8-one on melanosome transport and skin pigmentation.

Authors - Jong Il Park