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MOBP Polyclonal antibody

MOBP Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12472-1-AP

Synonyms

MOBP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using MOBP Polyclonal antibody (12472-1-AP)

    WB analysis of mouse brain using 12472-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12472-1-AP (MOBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MOBP Polyclonal antibody (12472-1-AP)

    IHC staining of human gliomas using 12472-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12472-1-AP (MOBP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MOBP Polyclonal antibody (12472-1-AP)

    IHC staining of human gliomas using 12472-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12472-1-AP (MOBP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MOBP Polyclonal antibody (12472-1-AP)

    IHC staining of mouse brain using 12472-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12472-1-AP (MOBP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MOBP Polyclonal antibody (12472-1-AP)

    IHC staining of mouse brain using 12472-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12472-1-AP (MOBP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12472-1-AP targets MOBP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MOBP fusion protein Ag3156
Full Name myelin-associated oligodendrocyte basic protein
Calculated Molecular Weight 183 aa, 21 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC022471
Gene Symbol MOBP
Gene ID (NCBI) 4336
RRIDAB_10644161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MOBP antibody 12472-1-APDownload protocol
IHC protocol for MOBP antibody 12472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neurobiol Dis

Inhibition of CXCR2 enhances CNS remyelination via modulating PDE10A/cAMP signaling pathway

Authors - Cheng Ju
View Article
mouseWB

Glia

ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons.

Authors - Lukasz Mateusz Szewczyk
View Article
humanIHC

Bioengineered

Four specific biomarkers associated with the progression of glioblastoma multiforme in older adults identified using weighted gene co-expression network analysis

Authors - Yushi Yang
View Article