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N-cadherin Antibody 10 Publications

Mouse Monoclonal| Catalog number: 66219-1-Ig |CloneNo.: 1D8B3

Featured Product KD/KO validated

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Con: 225 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

N-cadherin Antibody WB HEK-293 cells 66219-1-Ig

WB result of N-cadherin antibody (66219-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells

N-cadherin Antibody WB MKN-45 cells 66219-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

N-cadherin Antibody WB PC-3 cells 66219-1-Ig

Untreated and PNGase F-treated lysates of PC-3 cells were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

N-cadherin Antibody IF mouse heart tissue 66219-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

N-cadherin Antibody IF mouse heart tissue 66219-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

N-cadherin Antibody IHC mouse heart tissue 66219-1-Ig

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

N-cadherin Antibody IHC mouse heart tissue 66219-1-Ig

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, pig, mouse, rat
Positive WB detected in:
MKN-45 cells, HEK-293 cells, PC-3 cells, human heart tissue, pig brain tissue, rat brain tissue, SGC-7901 cells, ROS1728 cells, NIH/3T3 cells, C6 cells
Positive IHC detected in:
mouse heart tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse heart tissue
Recommended dilution:
WB : 1:2000-1:10000
IHC : 1:2000-1:10000
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
N-cadherin fusion protein Ag4996
Full name:
cadherin 2, type 1, N-cadherin (neuronal)
Calculated molecular weight:
906aa,100 kDa
Observed molecular weight:
130 kDa
GenBank accession number:
BC036470
Gene ID (NCBI):
1000
Gene symbol
CDH2
Synonyms
Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, N-cadherin, Neural cadherin

Background

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).


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