WB result of N-cadherin antibody (66219-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells

HEK-293 (N-cadherin+), PC-3 cells (N-cadherin+), C6 cells (N-cadherin+), NIH/3T3 cells (N-cadherin+), MCF-7 (N-cadherin-), A431 (N-cadherin-) cell lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

human heart tissue were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

Untreated and PNGase F-treated lysates of PC-3 cells were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

PC-3 cells were subjected to SDS PAGE followed by western blot with 66219-1-Ig( N-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

pig brain tissue were subjected to SDS PAGE followed by western blot with 66219-1-Ig( N-cadherin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

rat brain tissue were subjected to SDS PAGE followed by western blot with 66219-1-Ig( N-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunofluorescent analysis of ( 4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 11313-2-AP (alpha Actinin) in green.

Immunofluorescent analysis of ( 4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Immunofluorescent analysis of ( 4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)