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N-cadherin Monoclonal antibody

N-cadherin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat, rabbit

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D8B3

Cat no : 66219-1-Ig

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, N-cadherin, Neural cadherin



Tested Applications

Positive WB detected inrabbit brain tissue, pig brain tissue, human heart tissue, MKN-45 cells, HEK-293 cells, PC-3 cells, mouse brain tissue, rat brain tissue, C2C12 cells, C6 cells, Saos-2 cells, SGC-7901 cells, ROS1728 cells, NIH/3T3 cells
Positive IHC detected inmouse heart tissue, mouse brain tissue, human malignant melanoma tissue, human ovary tumor tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:7500-1:30000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66219-1-Ig targets N-cadherin in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse, rat, rabbit samples.

Tested Reactivity human, pig, mouse, rat, rabbit
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N-cadherin fusion protein Ag4996
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated Molecular Weight 906 aa, 100 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC036470
Gene Symbol CDH2
Gene ID (NCBI) 1000
RRIDAB_2881610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

Protocols

Product Specific Protocols
WB protocol for N-cadherin antibody 66219-1-IgDownload protocol
IHC protocol for N-cadherin antibody 66219-1-IgDownload protocol
IF protocol for N-cadherin antibody 66219-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.

Authors - Jiangfeng Li
humanWB

J Pineal Res

Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics

Authors - Ye Young Shin
humanWB,IHC

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
humanWB

Aging Cell

Advanced Maternal Age-associated SIRT1 Deficiency Compromises Trophoblast Epithelial-Mesenchymal Transition through an Increase in Vimentin Acetylation.

Authors - Liling Xiong
humanIF

Mater Today Bio

Ordered micropattern arrays fabricated by lung-derived dECM hydrogels for chemotherapeutic drug screening.

Authors - Xinglong Zhu
humanWB

Acta Biomater

Tumor cell membrane-based peptide delivery system targeting the tumor microenvironment for cancer immunotherapy and diagnosis.

Authors - Xiangzhou Meng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Davide (Verified Customer) (06-13-2023)

The antibody didn't produce any signal in 3d primary human cholangiocyte organoids. I would recommend either to try other antibody concentration or to try another Ab. It was tried on a cell condition which was recently reported to stimulate the CDH2 protein expression. However it should also be present in control condition at low levels.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cholangiocyte organoids
N-cadherin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:66219-1-Ig)
FH

Udesh (Verified Customer) (10-13-2022)

Used the Ab at 1:2000 dilution in 5% NFDM and observed a band at 140 kD. Blocked in 5% NFDM for 1H at RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: U2OS Osteosarcoma cells
N-cadherin Antibody Western Blot validation (1:2000 dilution) in U2OS Osteosarcoma cells (Cat no:66219-1-Ig)