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- KD/KO Validated
N-cadherin Monoclonal antibody
N-cadherin Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, mouse, rat
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
1D8B3
Cat no : 66219-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MKN-45 cells, HEK-293 cells, PC-3 cells, human heart tissue, pig brain tissue, rat brain tissue, SGC-7901 cells, ROS1728 cells, NIH/3T3 cells, C6 cells |
| Positive IHC detected in | mouse heart tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:10000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 24 publications below |
| IHC | See 4 publications below |
| IF | See 6 publications below |
Product Information
66219-1-Ig targets N-cadherin in WB, IHC, IF,ELISA applications and shows reactivity with human, pig, mouse, rat samples.
| Tested Reactivity | human, pig, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | N-cadherin fusion protein Ag4996 |
| Full Name | cadherin 2, type 1, N-cadherin (neuronal) |
| Calculated molecular weight | 906 aa, 100 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC036470 |
| Gene symbol | CDH2 |
| Gene ID (NCBI) | 1000 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for N-cadherin antibody 66219-1-Ig | Download protocol |
| IHC protocol for N-cadherin antibody 66219-1-Ig | Download protocol |
| IF protocol for N-cadherin antibody 66219-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Eur J Pharmacol Triptolide prevents proliferation and migration of Esophageal Squamous Cell Cancer via MAPK/ERK signaling pathway. | ||
Am J Transl Res VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro. | ||
J Exp Clin Cancer Res RUNX1 promotes tumour metastasis by activating the Wnt/β-catenin signalling pathway and EMT in colorectal cancer. | ||
Cancer Manag Res AOC1 Contributes to Tumor Progression by Promoting the AKT and EMT Pathways in Gastric Cancer. | ||
J Cell Mol Med METTL3/YTHDF2 m6 A axis promotes tumorigenesis by degrading SETD7 and KLF4 mRNAs in bladder cancer. |
