Tested Applications
| Positive WB detected in | HeLa cells, mouse spleen tissue, NIH/3T3 cells, C6 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human heart tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue |
| Positive IF/ICC detected in | HSkMC cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11313-2-AP targets Alpha Actinin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1859 Product name: Recombinant human a-Actinin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 543-892 aa of BC015766 Sequence: EIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL Predict reactive species |
| Full Name | actinin, alpha 1 |
| Calculated Molecular Weight | 103 kDa |
| Observed Molecular Weight | 100-105 kDa |
| GenBank Accession Number | BC015766 |
| Gene Symbol | Alpha Actinin |
| Gene ID (NCBI) | 87 |
| RRID | AB_2223815 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12814 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Alpha Actinin antibody 11313-2-AP | Download protocol |
| IF protocol for Alpha Actinin antibody 11313-2-AP | Download protocol |
| IHC protocol for Alpha Actinin antibody 11313-2-AP | Download protocol |
| WB protocol for Alpha Actinin antibody 11313-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Alpha Actinin (ACTN1) polyclonal antibody (Cat# 11313-2-AP) has accumulated 88 citations across journals including Nature Cell Biology, Nature Communications, Theranostics, Advanced Science, Cell Death & Differentiation, Science Advances, and FASEB Journal. Research fields span cardiology (hypertrophy, ischemia-reperfusion, heart failure), ferroptosis, oncology, cytoskeletal biology, immunology, neurology, ophthalmology, and tissue engineering, with applications primarily in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol TDP-43 skein-like inclusions are formed by BAG3- and HSP70-guided co-aggregation with actin-binding proteins | ||
J Extracell Vesicles Salvianolic Acid B Upregulates miR-744-5p Expression in Peripheral Blood Mononuclear Cell-Derived Extracellular Vesicles to Alleviate Uremic Cardiomyopathy. | ||
Nat Commun Medium from human iPSC-derived primitive macrophages promotes adult cardiomyocyte proliferation and cardiac regeneration | ||
ACS Nano Emerging of Ultrafine Membraneless Organelles as the Missing Piece of Nanostress: Mechanism of Biogenesis and Implications at Multilevels | ||
Redox Biol MK-886 protects against cardiac ischaemia/reperfusion injury by activating proteasome-Keap1-NRF2 signalling | ||
Theranostics An enzyme-mimicking reactive oxygen species scavenger targeting oxidative stress-inflammation cycle ameliorates IR-AKI by inhibiting pyruvate dehydrogenase kinase 4 |
Reviews
What customers say
Both reviewers gave this antibody a 5-star rating. One user reported excellent, consistent results in immunohistochemistry on formalin-fixed mouse myocardium at a 1/100 dilution. The other confirmed reliable performance in Western Blot on human umbilical vein tissue at a 1:1000 dilution, noting a clean 1:1 actinin band ratio. Overall, customers find this antibody highly specific and reproducible across applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jason (Verified Customer) (10-28-2019) | Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue.
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Steeve (Verified Customer) (01-08-2018) | Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin
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