N-cadherin Monoclonal antibody, PBS Only (Capture)

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  WB analysis of HEK-293 using 66219-1-Ig (same clone as 66219-1-PBS)

  WB result of N-cadherin antibody (66219-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  WB analysis using 66219-1-Ig (same clone as 66219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66219-1-Ig (same clone as 66219-1-PBS)

  Untreated and PNGase F-treated lysates of PC-3 cells were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  WB analysis of MKN-45 using 66219-1-Ig (same clone as 66219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  WB analysis using 66219-1-Ig (same clone as 66219-1-PBS)

  HEK-293 (N-cadherin+), PC-3 cells (N-cadherin+), C6 cells (N-cadherin+), NIH/3T3 cells (N-cadherin+), MCF-7 (N-cadherin-), A431 (N-cadherin-) cell lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66219-1-Ig (same clone as 66219-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66219-1-Ig (same clone as 66219-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 11313-2-AP (alpha Actinin) in green. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50439-2

  Cytometric bead array standard curve of MP50439-2, N-cadherin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66219-1-PBS. Detection antibody: 66219-2-PBS. Standard:Eg0701. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 66219-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66219-1-PBS against Human N-cadherin were performed. The affinity constant is 12.9 nM.

• Featured Product
• KD/KO Validated

N-cadherin Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 66219-1-PBS

Clone No.1D8B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

1D8B3, Cadherin 2, Cadherin-2, CD325, CDH2

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Product Information

66219-1-PBS targets N-cadherin as part of a matched antibody pair:

MP50439-2: 66219-1-PBS capture and 66219-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4996

  Product name: Recombinant human N-cadherin protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 33-350 aa of BC036470

  Sequence: KTGFPEDVYSAVLSKDVHEGQPLLNVKFSNCNGKRKVQYESSEPADFKVDEDGMVYAVRSFPLSSEHAKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVISPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQ

• Full Name: cadherin 2, type 1, N-cadherin (neuronal)
• Calculated Molecular Weight: 906 aa, 100 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC036470
• Gene Symbol: N-cadherin
• Gene ID (NCBI): 1000
• RRID: AB_2881610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P19022
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).