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- KD/KO Validated
NBR1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 16004-1-AP
1A1 3B, 1A13B, KIAA0049, M17S2, MIG19, NBR1, neighbor of BRCA1 gene 1, Next to BRCA1 gene 1 protein, Protein 1A1 3B
|Positive WB detected in||HeLa cells, A2780 cells, COS-7 cells, HepG2 cells, Jurkat cells, MCF-7 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse heart tissue, human breast cancer tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16004-1-AP targets NBR1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||dog, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NBR1 fusion protein Ag8652|
|Full Name||neighbor of BRCA1 gene 1|
|Calculated molecular weight||966aa,107 kDa|
|Observed molecular weight||140 kDa|
|GenBank accession number||BC009808|
|Gene ID (NCBI)||4077|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NBR1, also named as 1A13B, KIAA0049 and M17S2, acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. NBR1 and P62 can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. It is a highly conserved multidomain scaffold protein with proposed roles in endocytic trafficking and selective autophagy. NBR1 is a novel PB1 adapter in Th2 differentiation and asthma. It functions as an autophagy receptor involved in targeting ubiquitinated proteins for degradation. It also has a dual role as a scaffold protein to regulate growth-factor receptor and downstream signaling pathways. Observed MW of NBR1 is 140 kDa (PMID: 22654911, PMID: 22484440).
|Product Specific Protocols|
|WB protocol for NBR1 antibody 16004-1-AP||Download protocol|
|IHC protocol for NBR1 antibody 16004-1-AP||Download protocol|
|IF protocol for NBR1 antibody 16004-1-AP||Download protocol|
|IP protocol for NBR1 antibody 16004-1-AP||Download protocol|
|FC protocol for NBR1 antibody 16004-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The origin and role of autophagy in the formation of cytoplasmic granules in canine lingual granular cell tumors.
Cell Death Dis
The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy.
J Vet Med Sci
Electrophysiological and histopathological findings of muscular disease suspected as myotonic dystrophy in a Shiba dog.
Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.
Giant cell polymyositis and myocarditis associated with myasthenia gravis and thymoma.
Autophagy-related proteins (p62, NBR1 and LC3) in intranuclear inclusions in neurodegenerative diseases.