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NBR1 Antibody

NBR1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16004-1-AP

Synonyms

1A1 3B, 1A13B, KIAA0049, M17S2, MIG19, NBR1, neighbor of BRCA1 gene 1, Next to BRCA1 gene 1 protein, Protein 1A1 3B



Tested Applications

Positive WB detected inHeLa cells, A2780 cells, COS-7 cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue, human breast cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16004-1-AP targets NBR1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivity dog, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NBR1 fusion protein Ag8652
Full Name neighbor of BRCA1 gene 1
Calculated molecular weight 966aa,107 kDa
Observed molecular weight 140 kDa
GenBank accession number BC009808
Gene symbol NBR1
Gene ID (NCBI) 4077
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NBR1, also named as 1A13B, KIAA0049 and M17S2, acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. NBR1 and P62 can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. It is a highly conserved multidomain scaffold protein with proposed roles in endocytic trafficking and selective autophagy. NBR1 is a novel PB1 adapter in Th2 differentiation and asthma. It functions as an autophagy receptor involved in targeting ubiquitinated proteins for degradation. It also has a dual role as a scaffold protein to regulate growth-factor receptor and downstream signaling pathways. Observed MW of NBR1 is 140 kDa (PMID: 22654911, PMID: 22484440).

Protocols

Product Specific Protocols
WB protocol for NBR1 antibody 16004-1-APDownload protocol
IHC protocol for NBR1 antibody 16004-1-APDownload protocol
IF protocol for NBR1 antibody 16004-1-APDownload protocol
IP protocol for NBR1 antibody 16004-1-APDownload protocol
FC protocol for NBR1 antibody 16004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC, IF

Vet Pathol

The origin and role of autophagy in the formation of cytoplasmic granules in canine lingual granular cell tumors.

Authors - S Suzuki
humanWB,IP

Cell Death Dis

The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy.

Authors - Marie Chollat-Namy
dogIHC

J Vet Med Sci

Electrophysiological and histopathological findings of muscular disease suspected as myotonic dystrophy in a Shiba dog.

Authors - Takanori Shiga
humanIHC

Neuropathology

Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.

Authors - Fumiaki Mori
humanIHC

Neuropathology

Giant cell polymyositis and myocarditis associated with myasthenia gravis and thymoma.

Authors - Kon Tomoya T
humanIHC

Neurosci Lett

Autophagy-related proteins (p62, NBR1 and LC3) in intranuclear inclusions in neurodegenerative diseases.

Authors - Mori Fumiaki F