NCAM1/CD56 Monoclonal antibody

NCAM1/CD56 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E8C9

Cat no : 60238-1-Ig

Synonyms

CD56, MSK39, N CAM 1, NCAM, NCAM 1, NCAM1



Tested Applications

Positive WB detected infetal human brain tissue, C6 cells, human brain tissue, pig brain tissue
Positive IHC detected inhuman lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 60238-1-Ig is NCAM1/CD56 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, pig, rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NCAM1/CD56 fusion protein Ag5732
Full Name neural cell adhesion molecule 1
Calculated molecular weight 95 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC047244
Gene symbol NCAM1
Gene ID (NCBI) 4684
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurones, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

Protocols

Product Specific Protocols
WB protocol for NCAM1/CD56 antibody 60238-1-IgDownload protocol
IHC protocol for NCAM1/CD56 antibody 60238-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cells Dev

Effect of the Soluble Factors Released by Dental Apical Papilla-Derived Stem Cells on the Osteo/Odontogenic, Angiogenic, and Neurogenic Differentiation of Dental Pulp Cells.

Authors - Shi Yu