CoraLite® Plus 488-conjugated NCAM1/CD56 Monoclonal antibody

NCAM1/CD56 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E8C9

Cat no : CL488-60238

Synonyms

CD56, MSK39, N CAM 1, NCAM, NCAM 1, NCAM1, NCAM1/CD56

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Validation Data Gallery

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  IF Staining of SH-SY5Y using CL488-60238

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 NCAM1/CD56 antibody (CL488-60238, Clone: 1E8C9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60238 targets NCAM1/CD56 in IF applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NCAM1/CD56 fusion protein Ag5732
• Full Name: neural cell adhesion molecule 1
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC047244
• Gene Symbol: NCAM1
• Gene ID (NCBI): 4684
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).