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NDUFB5 Polyclonal antibody

NDUFB5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23855-1-AP

Synonyms

CI SGDH, Complex I SGDH, NDUFB5, SGDH

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using NDUFB5 Polyclonal antibody (23855-1-AP)

    WB analysis of mouse liver using 23855-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23855-1-AP (NDUFB5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using NDUFB5 Polyclonal antibody (23855-1-AP)

    WB analysis of mouse spleen using 23855-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 23855-1-AP (NDUFB5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDUFB5 Polyclonal antibody (23855-1-AP)

    IHC staining of human liver cancer using 23855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23855-1-AP (NDUFB5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDUFB5 Polyclonal antibody (23855-1-AP)

    IHC staining of human liver cancer using 23855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23855-1-AP (NDUFB5 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse liver tissue, mouse spleen tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23855-1-AP is NDUFB5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB5 fusion protein Ag20907
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa
Calculated Molecular Weight 189 aa, 22 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC005271
Gene Symbol NDUFB5
Gene ID (NCBI) 4711
RRIDAB_2879342
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NDUFB5 antibody 23855-1-APDownload protocol
IHC protocol for NDUFB5 antibody 23855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteome Sci

Effect of downregulated citrate synthase on oxidative phosphorylation signaling pathway in HEI-OC1 cells

Authors - Xiaowen Xu
View Article