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NEDD4 Polyclonal antibody

NEDD4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21698-1-AP

Synonyms

KIAA0093, NEDD 4, NEDD4, NEDD4 1, RPF1



Tested Applications

Positive WB detected inC6 cells, HEK-293 cells, HT-1080 cells, A549 cells, COS-7 cells, Jurkat cells
Positive IP detected inC6 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21698-1-AP is NEDD4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEDD4 fusion protein Ag16291
Full Name neural precursor cell expressed, developmentally down-regulated 4
Calculated molecular weight 1319 aa, 149 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC152452
Gene symbol NEDD4
Gene ID (NCBI) 4734
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEDD4-1(Neural precursor cell expressed developmentally down-regulated protein 4) is also named as NEDD4 and KIAA0093. It plays an essential role for the activation of the genetic program involved in the terminal maturation stage of invariant natural killer T-cell development(PMID:21949387). And it is differentially expressed in mammary epithelial cells and plays a role in luminal lineage determination(PMID:20351249). NEDD4-1 is a key regulator of FGFR1 endocytosis and signalling during neuronal differentiation and embryonic development(PMID:21765395). This antibody can recognize NEDD4-1 and NEDD4-2.

Protocols

Product Specific Protocols
WB protocol for NEDD4 antibody 21698-1-APDownload protocol
IHC protocol for NEDD4 antibody 21698-1-APDownload protocol
IP protocol for NEDD4 antibody 21698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.

Authors - Xi Shen
humanWB

J Exp Clin Cancer Res

N-myc downstream-regulated gene 1 inhibits the proliferation of colorectal cancer through emulative antagonizing NEDD4-mediated ubiquitylation of p21.

Authors - Sen Zhang
humanWB

Biochem Biophys Res Commun

O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway.

Authors - Kuan Jiang
ratWB

Life Sci

Inhibition of ubiquitin proteasome function prevents monocrotaline-induced pulmonary arterial remodeling.

Authors - Yanting Zhu
WB

Virus Res

Multivesicular body sorting and the exosomal pathway are required for the release of rat hepatitis E virus from infected cells.

Authors - Putu Prathiwi Primadharsini
humanWB

Exp Cell Res

NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.

Authors - Jianning Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-25-2020)

This NEDD4 antibody works very well in western blots.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart