|Positive WB detected in||C6 cells, HEK-293 cells, HT-1080 cells, A549 cells, COS-7 cells, Jurkat cells|
|Positive IP detected in||C6 cells|
|Positive IHC detected in||human breast cancer tissue, human lung cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21698-1-AP is NEDD4 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NEDD4 fusion protein Ag16291|
|Full Name||neural precursor cell expressed, developmentally down-regulated 4|
|Calculated molecular weight||1319 aa, 149 kDa|
|Observed molecular weight||115 kDa|
|GenBank accession number||BC152452|
|Gene ID (NCBI)||4734|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NEDD4-1(Neural precursor cell expressed developmentally down-regulated protein 4) is also named as NEDD4 and KIAA0093. It plays an essential role for the activation of the genetic program involved in the terminal maturation stage of invariant natural killer T-cell development(PMID:21949387). And it is differentially expressed in mammary epithelial cells and plays a role in luminal lineage determination(PMID:20351249). NEDD4-1 is a key regulator of FGFR1 endocytosis and signalling during neuronal differentiation and embryonic development(PMID:21765395). This antibody can recognize NEDD4-1 and NEDD4-2.
Biochem Biophys Res Commun
YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.
J Exp Clin Cancer Res
N-myc downstream-regulated gene 1 inhibits the proliferation of colorectal cancer through emulative antagonizing NEDD4-mediated ubiquitylation of p21.
Biochem Biophys Res Commun
O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway.
Inhibition of ubiquitin proteasome function prevents monocrotaline-induced pulmonary arterial remodeling.
Multivesicular body sorting and the exosomal pathway are required for the release of rat hepatitis E virus from infected cells.
Exp Cell Res
NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (08-25-2020)
This NEDD4 antibody works very well in western blots.