NF-L Antibody 0 Publications

Mouse Monoclonal| Catalog number: 60189-1-Ig |CloneNo.: 5C12G4

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Con: 210 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
Human stomach, tissue

Positive IHC detected in:
human brain tissue

Positive IF detected in:
mouse brain tissue

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information


Purification method:
Protein G purification


PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Full name:
neurofilament, light polypeptide

Calculated molecular weight:
543aa,62 kDa

Observed molecular weight:
65 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol

68 kDa neurofilament protein, NEFL, Neurofilament L, NF68, NFL, NF-L

NEFL, also named as NF68 and NFL, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd.This antibody is specific to NEFL. It has a very weak reaction to NEFM.

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