NF-L Monoclonal antibody

NF-L Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5C12G4

Cat no : 60189-1-Ig

Synonyms

68 kDa neurofilament protein, NEFL, Neurofilament L, NF, NF68, NFL, NF-L



Tested Applications

Positive WB detected inPC-12 cells
Positive IHC detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60189-1-Ig targets NF-L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NF-L fusion protein Ag15178
Full Name neurofilament, light polypeptide
Calculated molecular weight 543 aa, 62 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC039237
Gene symbol NEFL
Gene ID (NCBI) 4747
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL.

Protocols

Product Specific Protocols
WB protocol for NF-L antibody 60189-1-IgDownload protocol
IHC protocol for NF-L antibody 60189-1-IgDownload protocol
IF protocol for NF-L antibody 60189-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols