NF-L Monoclonal antibody, PBS Only

NF-L Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C12G4

Cat no : 60189-1-PBS

Synonyms

68 kDa neurofilament protein, NEFL, Neurofilament L, NF, NF68, NFL, NF-L

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Validation Data Gallery

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  WB analysis using 60189-1-Ig (same clone as 60189-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60189-1-Ig (NF-L antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 60189-1-Ig (same clone as 60189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60189-1-Ig (same clone as 60189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 60189-1-Ig (same clone as 60189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 60189-1-Ig (same clone as 60189-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60189-1-Ig (NF-L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 60189-1-Ig (same clone as 60189-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-L antibody (60189-1-Ig, Clone: 5C12G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-12 using 60189-1-Ig (same clone as 60189-1-PBS)

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-L (60189-1-Ig, Clone:5C12G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60189-1-PBS targets NF-L in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NF-L fusion protein Ag15178
• Full Name: neurofilament, light polypeptide
• Calculated Molecular Weight: 543 aa, 62 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC039237
• Gene Symbol: NEFL
• Gene ID (NCBI): 4747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL.