CoraLite® Plus 488-conjugated NF-L Monoclonal antibody
NF-L Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5C12G4
Cat no : CL488-60189
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-60189 targets NF-L in IF applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | NF-L fusion protein Ag15178 |
Full Name | neurofilament, light polypeptide |
Calculated molecular weight | 543 aa, 62 kDa |
GenBank accession number | BC039237 |
Gene symbol | NEFL |
Gene ID (NCBI) | 4747 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 NF-L antibody CL488-60189 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |