CoraLite® Plus 488-conjugated NF-L Monoclonal antibody

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  IF Staining of mouse brain using CL488-60189

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 NF-L antibody (CL488-60189, Clone: 5C12G4 ) at dilution of 1:200.

NF-L Monoclonal Antibody for IF-P

Cat No. CL488-60189

Clone No.5C12G4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P

Synonyms

68 kDa neurofilament protein, CMT1F, CMT2E, FLJ53642, NEFL, neurofilament, NF, NF L, NF68, NFL, NF-L

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60189 targets NF-L in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NF-L fusion protein Ag15178
• Full Name: neurofilament, light polypeptide
• Calculated Molecular Weight: 543 aa, 62 kDa
• GenBank Accession Number: BC039237
• Gene Symbol: NF-L
• Gene ID (NCBI): 4747
• RRID: AB_2883115
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P07196
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.