CoraLite® Plus 488-conjugated NF-L Monoclonal antibody

NF-L Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5C12G4

Cat no : CL488-60189

Synonyms

68 kDa neurofilament protein, CMT1F, CMT2E, FLJ53642, NEFL, neurofilament, NF, NF L, NF68, NFL, NF-L

---

Validation Data Gallery

• 

  IF Staining of mouse brain using CL488-60189

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 NF-L antibody (CL488-60189, Clone: 5C12G4 ) at dilution of 1:200.

---

Tested Applications

• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL488-60189 targets NF-L in IF applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NF-L fusion protein Ag15178
• Full Name: neurofilament, light polypeptide
• Calculated molecular weight: 543 aa, 62 kDa
• GenBank accession number: BC039237
• Gene symbol: NEFL
• Gene ID (NCBI): 4747
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.