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CoraLite®594-conjugated NF-L Monoclonal antibody

NF-L Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5C12G4

Cat no : CL594-60189

Synonyms

68 kDa neurofilament protein, CMT1F, CMT2E, FLJ53642, NEFL, Neurofilament L, NF, NF L, NF68, NFL, NF-L

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated NF-L Monoclonal antibody (CL594-60189)

    IF Staining of mouse brain using CL594-60189

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 NF-L antibody (CL594-60189, Clone: 5C12G4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite®594-conjugated NF-L Monoclonal antibody (CL594-60189)

    FC experiment of SH-SY5Y using CL594-60189

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NF-L (CL594-60189, Clone:5C12G4) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inmouse brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60189 targets NF-L in IF, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NF-L fusion protein Ag15178
Full Name neurofilament, light polypeptide
Calculated Molecular Weight 543 aa, 62 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC039237
Gene Symbol NEFL
Gene ID (NCBI) 4747
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL.

Protocols

Product Specific Protocols
IF protocol for CL594 NF-L antibody CL594-60189Download protocol
Standard Protocols
Click here to view our Standard Protocols