|Positive WB detected in||rat brain tissue, human brain tissue, human cerebellum tissue|
|Positive IHC detected in||human brain tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:350-1:1400|
|Sample-dependent, check data in validation data gallery|
66513-1-Ig targets OLIG2 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||OLIG2 fusion protein Ag18838|
|Full Name||oligodendrocyte lineage transcription factor 2|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||35-39 kDa|
|GenBank accession number||BC047511|
|Gene ID (NCBI)||10215|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )
Brain Res Bull
Matrine protects oligodendrocytes by inhibiting their apoptosis and enhancing mitochondrial autophagy.
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Single-Cell RNA Sequencing With Combined Use of Bulk RNA Sequencing to Reveal Cell Heterogeneity and Molecular Changes at Acute Stage of Ischemic Stroke in Mouse Cortex Penumbra Area.