OLIG2 Monoclonal antibody

OLIG2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E8C5

Cat no : 66513-1-Ig

Synonyms

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17



Tested Applications

Positive WB detected inrat brain tissue, human brain tissue, human cerebellum tissue
Positive IHC detected inhuman brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:350-1:1400
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

66513-1-Ig targets OLIG2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen OLIG2 fusion protein Ag18838
Full Name oligodendrocyte lineage transcription factor 2
Calculated molecular weight 32 kDa
Observed molecular weight 35-39 kDa
GenBank accession numberBC047511
Gene symbol OLIG2
Gene ID (NCBI) 10215
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )

Protocols

Product Specific Protocols
WB protocol for OLIG2 antibody 66513-1-IgDownload protocol
IHC protocol for OLIG2 antibody 66513-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Brain Res Bull

Matrine protects oligodendrocytes by inhibiting their apoptosis and enhancing mitochondrial autophagy.

Authors - Meng-Ru Wang