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OPTN Polyclonal antibody

OPTN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10837-1-AP

Synonyms

E3 14.7K interacting protein, FIP 2, FIP2, GLC1E, HIP 7, HIP7, Huntingtin yeast partner L, HYPL, NEMO related protein, NRP, optineurin, OPTN, TFIIIA INTP



Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10837-1-AP targets OPTN in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPTN fusion protein Ag1272
Full Name optineurin
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa, 70-74 kDa
GenBank accession numberBC013876
Gene symbol OPTN
Gene ID (NCBI) 10133
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for OPTN antibody 10837-1-APDownload protocol
IHC protocol for OPTN antibody 10837-1-APDownload protocol
IF protocol for OPTN antibody 10837-1-APDownload protocol
IP protocol for OPTN antibody 10837-1-APDownload protocol
FC protocol for OPTN antibody 10837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging.

Authors - Davide D'Amico
WB

Nat Commun

A selective inhibitor of mitofusin 1-βIIPKC association improves heart failure outcome in rats.

Authors - Julio C B Ferreira
humanWB

Oncogene

Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab.

Authors - Dawei Wang
humanWB

Mol Genet Genomic Med

Association of the TBK1 mutation p.Ile334Thr with frontotemporal dementia and literature review.

Authors - Huiling Yu
mouseWB

EMBO Rep

Parkin controls brown adipose tissue plasticity in response to adaptive thermogenesis.

Authors - Montserrat Cairó
humanIF

Elife

Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology.

Authors - Peipei Zhang