Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, pig skeletal muscle tissue |
| Positive IHC detected in | human pancreas cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60293-1-Ig targets OPTN/Optineurin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1272 Product name: Recombinant human OPTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 233-577 aa of BC013876 Sequence: VSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII Predict reactive species |
| Full Name | optineurin |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 70-74 kDa |
| GenBank Accession Number | BC013876 |
| Gene Symbol | OPTN |
| Gene ID (NCBI) | 10133 |
| RRID | AB_2881408 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96CV9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OPTN/Optineurin antibody 60293-1-Ig | Download protocol |
| IHC protocol for OPTN/Optineurin antibody 60293-1-Ig | Download protocol |
| WB protocol for OPTN/Optineurin antibody 60293-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OPTN/Optineurin Monoclonal antibody (6C1H4), catalog number 60293-1-Ig, has 4 citations. These appear in Autophagy, Developmental Cell, F1000Research, and Gut Microbes. Research fields span copper-dependent autophagic degradation and ferroptosis, mitophagy in glioblastoma stem cells, antibody validation for Optineurin, and diabetes-associated cognitive decline involving neuronal mitophagy. Applications cited include WB, IF, and IP in human samples.
| Species | Application | Title |
|---|---|---|
Dev Cell PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.
| ||
F1000Res A guide to selecting high-performing antibodies for Optineurin (UniProt ID: Q96CV9) for use in western blot, immunoprecipitation, and immunofluorescence.
| ||
Gut Microbes Akkermansia muciniphila protects diabetes-associated cognitive decline by enhancing leucine catabolism and suppressing neuronal excessive mitophagy. |
Reviews
What customers say
The product has one verified review. The reviewer used it for Western Blot at a 1:4000 dilution on iPSCs and awarded a perfect 5-star rating, confirming reliable performance in their application. No written comments were provided, but the high score and inclusion of a representative blot image indicate satisfactory results with this antibody.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Malika (Verified Customer) (10-02-2025) |
 |
