OPTN/Optineurin Monoclonal antibody, PBS Only

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  WB analysis using 60293-1-Ig (same clone as 60293-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60293-1-Ig (OPTN/Optineurin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60293-1-Ig (same clone as 60293-1-PBS)

  WB result of OPTN/Optineurin antibody (60293-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPTN/Optineurin transfected HeLa cells. This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60293-1-Ig (same clone as 60293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60293-1-Ig (same clone as 60293-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 60293-1-Ig (same clone as 60293-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OPTN antibody (60293-1-Ig, Clone: 6C1H4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

OPTN/Optineurin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 60293-1-PBS

Clone No.6C1H4

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Optineurin, OPTN, 6C1H4, ALS12, E3-14.7K-interacting protein

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Product Information

60293-1-PBS targets OPTN/Optineurin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1272

  Product name: Recombinant human OPTN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 233-577 aa of BC013876

  Sequence: VSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII

• Full Name: optineurin
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 70-74 kDa
• GenBank Accession Number: BC013876
• Gene Symbol: OPTN
• Gene ID (NCBI): 10133
• RRID: AB_2881408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96CV9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.