P2RY1 Monoclonal antibody
P2RY1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Pig, Mouse, Rat, Rabbit and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1A1F3
Cat no : 67654-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue, rabbit brain tissue |
| Positive IHC detected in | mouse brain tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | rat brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
67654-1-Ig targets P2RY1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.
| Tested Reactivity | Human, Pig, Mouse, Rat, Rabbit |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | P2RY1 fusion protein Ag12762 |
| Full Name | purinergic receptor P2Y, G-protein coupled, 1 |
| Calculated Molecular Weight | 373 aa, 42 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC074785 |
| Gene Symbol | P2RY1 |
| Gene ID (NCBI) | 5028 |
| RRID | AB_2882853 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P2RY1 antibody 67654-1-Ig | Download protocol |
| IHC protocol for P2RY1 antibody 67654-1-Ig | Download protocol |
| IF protocol for P2RY1 antibody 67654-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci China Life Sci Inflammatory macrophages exacerbate neutrophil-driven joint damage through ADP/P2Y1 signaling in rheumatoid arthritis. | ||
Oncol Lett New prognostic factors and scoring system for patients with acute myeloid leukemia. |
