Palladin Antibody 44 Publications

Rabbit Polyclonal| Catalog number: 10853-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 32 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
HeLa cells, A549 cells

Positive IP detected in:
HeLa cells, HEK-293 cells

Positive IHC detected in:
human colon cancer tissue, human breast cancer tissue, human colon tissue, human colon cancer tissue, human ovary tumor tissue

Positive IF detected in:
HeLa cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
palladin, cytoskeletal associated protein

Calculated molecular weight:
1383aa, 151 kDa

Observed molecular weight:
95 kDa, 140 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
PALLD

Synonyms
CGI 151, FLJ22190, FLJ38193, FLJ39139, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002
Background

Palladin is an actin associated protein serving as a cytoskeleton scaffold, and actin cross linker, localizing at stress fibers, focal adhesions, and other actin based structures. Palladin exists as multiple isoforms through alternative transcription initiation sites and splicing. There are three major isoforms (200, 140, 90-92 kDa) and multiple minor isoforms. Different palladin isoforms are expressed in a tissue-specific pattern during development and in adult organs: the 200 kDa isoform is predominantly expressed in the heart, skeletal muscle, testis and bone; the 140 kDa isoform is widely expressed with the exception of liver, muscle, and skin; the 90-92 kDa isoform is broadly expressed in embryonic tissues and highly expressed in adult smooth muscle tissues (21455759). Recently overexpression of palladin has been linked to the promoted invasive motility in several types of cancers (18978809, 22291919). The 85-90 kDa palladin isoform has been observed predominantly expressed in pancreatic ductal adenocarcinoma (PDA) while a 65 kDa isoform is expressed in normal pancreas and non-PDA tumors (20436683). This antibody, generated against the fragment 999-1383aa of palladin, recognizes most isoforms of this protein, except isoform 6.


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