CoraLite®555-conjugated Palladin Polyclonal antibody

Palladin Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG


human, mouse


IF, FC (Intra)


CoraLite®555 Fluorescent Dye

Cat no : CL555-10853


CGI 151, FLJ22190, FLJ38193, FLJ39139, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002

Tested Applications

Positive IF detected inmouse testis tissue
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10853-1-AP

Recommended dilution

Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL555-10853 targets Palladin in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Palladin fusion protein Ag1288
Full Name palladin, cytoskeletal associated protein
Calculated molecular weight 1383 aa, 151 kDa
Observed molecular weight 95 kDa, 140 kDa
GenBank accession numberBC013867
Gene symbol PALLD
Gene ID (NCBI) 23022
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths555 nm / 570 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Palladin is an actin associated protein serving as a cytoskeleton scaffold, and actin cross linker, localizing at stress fibers, focal adhesions, and other actin based structures. Palladin exists as multiple isoforms through alternative transcription initiation sites and splicing. There are three major isoforms (200, 140, 90-92 kDa) and multiple minor isoforms. Different palladin isoforms are expressed in a tissue-specific pattern during development and in adult organs: the 200 kDa isoform is predominantly expressed in the heart, skeletal muscle, testis and bone; the 140 kDa isoform is widely expressed with the exception of liver, muscle, and skin; the 90-92 kDa isoform is broadly expressed in embryonic tissues and highly expressed in adult smooth muscle tissues (21455759). Recently overexpression of palladin has been linked to the promoted invasive motility in several types of cancers (18978809, 22291919). The 85-90 kDa palladin isoform has been observed predominantly expressed in pancreatic ductal adenocarcinoma (PDA) while a 65 kDa isoform is expressed in normal pancreas and non-PDA tumors (20436683). This antibody, generated against the fragment 999-1383aa of palladin, recognizes most isoforms of this protein, except isoform 6.


Product Specific Protocols
IF protocol for CL555 Palladin antibody CL555-10853Download protocol
FC protocol for CL555 Palladin antibody CL555-10853Download protocol
Standard Protocols
Click here to view our Standard Protocols